Activation of a Lotus japonicus subtilase gene during arbuscular mycorrhiza is dependent on the common symbiosis genes and two cis-active promoter regions.
Activation of a Lotus japonicus subtilase gene during arbuscular mycorrhiza is dependent on the common symbiosis genes and two cis-active promoter regions.
复制标题
DOI：
10.1094/mpmi-09-10-0220
复制
发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
N. Takeda;K. Haage;Shusei Sato;S. Tabata;M. Parniske
中科院分区：
文献类型：
--
作者：
N. Takeda;K. Haage;Shusei Sato;S. Tabata;M. Parniske
在丛枝菌根(AM)共生过程中，类枯草杆菌丝氨酸蛋白酶SbtM1被强烈而特异地诱导。另一种枯草杆菌酶基因SBTS在结瘤和AM的早期阶段被诱导植物共生突变体的转录谱分析表明，AM诱导的SbtM1及其基因家族成员SbtM3和SbtM4的表达依赖于共同的共生途径，而SBTS的激活则是一条独立的途径。我们使用SbtM1启动子β-d-葡萄糖醛酸酶(GUS)融合的特定空间表达模式来分离赋予AM反应性的顺式元件。启动子缺失和替换分析确定了SbtM1启动子中的两个顺式区域(区域I和II)，这是AM诱导GUS活性所必需的。35S最小启动子融合显示，在串联重复序列中测试时，这两个区域中的任何一个都足以满足AM的反应性。在紫花苜蓿和水稻中发现了AM诱导枯草杆菌酶基因启动子的序列相关区域，这与被子植物分化之前这些元件的古老起源是一致的。
The subtilisin-like serine protease SbtM1 is strongly and specifically induced during arbuscular mycorrhiza (AM) symbiosis in Lotus japonicus. Another subtilase gene, SbtS, is induced during early stages of nodulation and AM. Transcript profiling in plant symbiosis mutants revealed that the AM-induced expression of SbtM1 and the gene family members SbtM3 and SbtM4 is dependent on the common symbiosis pathway, whereas an independent pathway contributes to the activation of SbtS. We used the specific spatial expression patterns of SbtM1 promoter β-d-glucuronidase (GUS) fusions to isolate cis elements that confer AM responsiveness. A promoter deletion and substitution analysis defined two cis regions (region I and II) in the SbtM1 promoter necessary for AM-induced GUS activity. 35S minimal promoter fusions revealed that either of the two regions is sufficient for AM responsiveness when tested in tandem repeat arrangement. Sequence-related regions were found in the promoters of AM-induced subtilase genes in Medicago truncatula and rice, consistent with an ancient origin of these elements predating the divergence of the angiosperms.