Delay in synthesis of the 3' splice site promotes trans-splicing of the preceding 5' splice site.
Delay in synthesis of the 3' splice site promotes trans-splicing of the preceding 5' splice site.
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DOI：
10.1016/j.molcel.2005.03.018
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发表时间：
2005-04
期刊：
影响因子：
16
通讯作者：
Terunao Takahara;Bosiljka Tasic;T. Maniatis;H. Akanuma;S. Yanagisawa
中科院分区：
文献类型：
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作者：
Terunao Takahara;Bosiljka Tasic;T. Maniatis;H. Akanuma;S. Yanagisawa
Premessenger RNA (pre-mRNA) splicing can occur within an individual pre-mRNA (cis-splicing) or between separate pre-mRNAs (trans-splicing). Although a number of examples of mammaliantrans-splicing have been reported, the molecular mechanisms involved are poorly understood. Here, we investigate the mechanisms of Sp1 pre-mRNAtrans-splicing with human cells expressing modifiedSp1transgenes. We find that the presence of a long intron or the insertion of an RNA polymerase II pause site within an intron promotestrans-splicing. We also add examples of naturally occurringtrans-splicing. We propose that Sp1trans-splicing, and other examples of mammaliantrans-splicing, are a consequence of low-frequency disruption of the normal mechanisms that couple transcription and splicing.
