Bone response to the multilayer BMP-2 gene coated porous titanium implant surface
Bone response to the multilayer BMP-2 gene coated porous titanium implant surface
复制标题
多层 BMP-2 基因涂层多孔钛种植体表面的骨反应
DOI：
10.1111/j.1600-0501.2011.02383.x
复制
发表时间：
2013-08-01
影响因子：
4.3
通讯作者：
He, Fu-Ming
中科院分区：
文献类型：
--
作者：
Jiang, Qiao-Hong;Liu, Li;He, Fu-Ming
目的：评估体外喷砂钛上形成的质粒多层基因传递和体内类似制备的种植体表面周围骨形成后hBMP-2的表达。材料与方法：将多层阳离子脂质/rhBMP-2质粒DNA复合物（LDc）和阴离子透明质酸（HA）采用分层组装（LBL）方法组装在喷砂双酸蚀刻纯钛盘或种植体表面。体外检测暴露于LDc多层膜的细胞中的基因传递和hBMP-2表达。为了确定BMP-2 LDc多层膜在体内对BMP传递的影响，我们将涂有BMP-2 LDc多层膜的粗糙植入物或未涂有BMP-2 LDc多层膜的对照植入物（两种植入物各15只）植入NZW兔的股骨。2、4、8周后取出股骨，准备进行组织形态学评估（每个时间点n = 5只兔）。结果：MC3T3-E1细胞直接在BMP-2 LDc包覆钛盘上培养48 h后，EGFP和hBMP-2均有表达。当细胞培养48小时时，通过增加组装层数，基因传递增加。在LDc包被表面上培养的细胞比在未包被多孔钛表面培养的对照细胞具有显著更高的细胞活力。组织学观察显示，愈合4周后，LDc涂层表面的骨与种植体接触（BIC）明显低于对照表面，但未达到显著性水平。相比之下，种植体螺纹内的骨百分比显著高于对照组（P = 0.047）。结论：BMP-2基因包被喷砂双酸蚀钛种植体对种植体周围早期骨形成有一定的促进作用。
Objectives: Evaluate hBMP-2 expression following gene delivery from plasmid multilayers formed on sandblasted titanium in vitro and bone formation around similarly prepared implant surfaces in vivo.Materials and methods: Multilayers of cationic lipid/rhBMP-2 plasmid DNA complex (LDc) and anionic hyaluronic acid (HA) was assembled on sandblasted-dual acid etched pure titanium disks or implant surfaces using layer-by-layer (LBL) assembly. Gene delivery and hBMP-2 expression in cells exposed to the LDc multilayers was measured in vitro. To determine the effect of BMP delivery from such multilyaers in vivo, roughened implants coated with BMP-2 LDc multilayers or uncoated control implants (n = 15 for both) were implanted in the femurs of NZW rabbits. After 2, 4, 8 weeks, femurs were retrieved and prepared for histomorphometric evaluation (n = 5 rabbits per time point).Results: MC3T3-E1 cells cultured directly on the BMP-2 LDc coated titanium disks showed EGFP and hBMP-2 expression after 48 h in culture. Increased gene delivery occurred by increasing the number of assembly layers when cells were cultured for 48 h. Cells cultured on LDc coated surfaces had significantly higher cell viability than control cells cultured on uncoated porous titanium surfaces. Histologic observation of the implants showed that after 4 weeks healing, the bone to implant contact (BIC) on the LDc coated surface was much lower than that on the control surface, but didn't reach significant. In contrast, the percentage of bone within the implant's threads was significantly higher than the control group (P = 0.047).Conclusion: The BMP-2 gene coated sandblasted dual acid etched titanium implants slightly accelerated early bone formation around implants.