Expression profiles of inka2 in the murine nervous system
Expression profiles of inka2 in the murine nervous system
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DOI：
10.1016/j.gep.2015.08.002
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发表时间：
2015-09-01
影响因子：
1.2
通讯作者：
Sakakibara, Shin-ichi
中科院分区：
文献类型：
--
作者：
Iwasaki, Yumi;Yumoto, Takahito;Sakakibara, Shin-ichi
肌动蛋白细胞骨架的动态重排影响发育中和成人中枢神经系统（CNS）的许多细胞特征，包括高活动性神经祖细胞的迁移和粘附、未成熟神经元的轴突引导以及成人大脑中突触结构的重建。 Inka1 是一种已知的肌动蛋白细胞骨架重建调节因子，主要由神经嵴细胞谱系表达，并调节这些细胞的迁移和分化。在本研究中，我们鉴定了一个新基因，命名为 inka2，它与 inka1 相关。 Inka2/fam212b是在不同脊椎动物物种中发现的进化保守基因，与inka1一起构成一个新的基因家族。 Northern blot分析表明inka2 mRNA在神经系统中高度富集。表达 inka2 转录物的那些细胞的时空繁殖细胞谱与 Olig2 阳性少突胶质细胞祖细胞的时空繁殖细胞谱相一致，后者在胚胎发生过程中起源于腹侧心室区。在发育中的小脑的增殖性神经元祖细胞中也发现了 inka2 的强烈表达。另一方面，胚胎脑中未成熟的新生神经元不表达inka2，除了位于胚胎端脑边缘区的细胞外，已知该区域含有瞬态细胞，包括非亚板先驱神经元和CajaleRetzius细胞。随着出生后大脑发育的进行，inka2在一些未成熟神经元群体中出现表达，包括新皮质锥体神经元、海马锥体神经元和在小脑皮质中迁移的颗粒细胞。有趣的是，在成人大脑中，inka2 的表达被限制在受限前脑区域的终末分化神经元中。总而言之，作为中枢神经系统肌动蛋白细胞骨架的新型调节因子，inka2 可能参与多种肌动蛋白驱动的过程，包括细胞迁移和神经元极性的建立。 (C) 2015 Elsevier B.V. 保留所有权利。
Dynamic rearrangement of the actin cytoskeleton impacts many cellular characteristics in both the developing and adult central nervous systems (CNS), including the migration and adhesion of highly motile neural progenitor cells, axon guidance of immature neurons, and reconstruction of synaptic structures in the adult brain. Inka1, a known regulator of actin cytoskeleton reconstruction, is predominantly expressed by the neural crest cell lineage and regulates the migration and differentiation of these cells. In the present study, we identified a novel gene, designated as inka2, which is related to inka1. Inka2/fam212b is an evolutionarily conserved gene found in different vertebrate species and constitutes a novel gene family together with inka1. Northern blot analysis showed that inka2 mRNA was highly enriched in the nervous system. The spatiotemporal propagation cell profiles of those cells that expressed inka2 transcripts were compatible with those of Olig2-positive oligodendrocyte progenitor cells, which originate in the ventral ventricular zone during embryogenesis. Intense expression of inka2 was also noted in the proliferative neuronal progenitors in the developing cerebellum. On the other hand, immature newborn neurons in the embryonic brain showed no expression of inka2, except for the cells residing in the marginal zone of the embryonic telencephalon, which is known to contain transient cells including the non-subplate pioneer neurons and CajaleRetzius cells. As brain development proceeds during the postnatal stage, inka2 expression emerged in some populations of immature neurons, including the neocortical pyramidal neurons, hippocampal pyramidal neurons, and granule cells migrating in the cerebellar cortex. In the adult brain, the expression of inka2 was interestingly confined in terminally differentiated neurons in the restricted forebrain regions. Taken together, as a novel regulator of actin cytoskeletons in the CNS, inka2 may be involved in multiple actin-driven processes, including cell migration and establishment of neuronal polarity. (C) 2015 Elsevier B.V. All rights reserved.