Prevalence of avian malaria parasite in mosquitoes collected at a zoological garden in Japan
Prevalence of avian malaria parasite in mosquitoes collected at a zoological garden in Japan
复制标题
DOI：
10.1007/s00436-009-1434-9
复制
发表时间：
2009-09-01
影响因子：
2
通讯作者：
Yukawa, Masayoshi
中科院分区：
文献类型：
--
作者：
Ejiri, Hiroko;Sato, Yukita;Yukawa, Masayoshi
日本动物园饲养的几种鸟类被发现感染了禽疟原虫。然而，尚未确定受牵连的病媒蚊子种类。为了表明禽疟原虫的主管载体，我们在日本的动物园收集蚊子，并检查了禽疟原虫DNA。2005年在日本东京以南的神奈川动物园共采集到11种蚊虫1,361只。捕获的蚊子按每个物种、收集日期和位置合并，并用于DNA提取。169份DNA中8份为鸡疟原虫cyt B基因巢式PCR阳性。估计蚊子的最低感染率为千分之5.9。PCR阳性蚊种为尖音库蚊组和嗜食Lutzia vorax。从收集的蚊子中扩增的一些DNA序列与从研究的同一动物园中捕获的鸟类中检测到的鸟类疟原虫谱系相同。我们的结果表明C. pipiens组和L. vorax可能是日本动物园圈养鸟类传播禽疟原虫的主要媒介。
Several species of captive birds at zoological gardens of Japan were found to be infected with avian Plasmodium. However, incriminated vector mosquito species have not been identified yet. To indicate the competent vectors of avian malaria parasite, we collected mosquitoes at a zoological garden in Japan and examined for the avian malaria parasite DNA. Totally, 1,361 mosquitoes of 11 species were collected in the zoological garden of Kanagawa, the south of Tokyo in Japan in 2005. Captured mosquitoes were pooled by each species, date collected, and location and used for DNA extraction. Eight out of 169 DNA samples were positive for the nested PCR of avian Plasmodium cyt b gene. Estimated minimum infection rates of mosquitoes were 5.9 per 1,000. The PCR positive mosquito species were Culex pipiens group and Lutzia vorax. Some DNA sequences amplified from collected mosquitoes were identical to avian Plasmodium lineages detected from captive birds in the same zoological garden studied. Our results suggest that C. pipiens group and L. vorax could be incriminated vectors of avian malaria parasite transmitting in captive birds kept in the zoological garden in Japan.