CHARACTERIZATION OF THE INFLAMMATORY REACTION IN THE PERIPHERAL AIRWAYS OF CIGARETTE SMOKERS USING IMMUNOCYTOCHEMISTRY
CHARACTERIZATION OF THE INFLAMMATORY REACTION IN THE PERIPHERAL AIRWAYS OF CIGARETTE SMOKERS USING IMMUNOCYTOCHEMISTRY
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DOI：
10.1164/ajrccm/145.4_pt_1.911
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发表时间：
1992-04-01
期刊：
影响因子：
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通讯作者：
HOGG, JC
中科院分区：
文献类型：
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作者：
BOSKEN, CH;HARDS, J;HOGG, JC
采用单克隆抗体研究了20例外周癌切除术患者的肺组织，以确定外周气道中的炎性细胞类型，并确定支气管相关淋巴组织（BALT）的位置。根据FEV 1预测值百分比（%FEV1）将患者分为阻塞组（%FEV1 < 80%）和对照组（%FEV1 > 80%）。将切除的肺填充稀释的冷冻包埋介质（Tissue-Tek R），在液氮上冷冻，使用带锯切成2 cm矢状切片，并使用软木孔取样。从这些样本中切下10个连续的组织切片，用特异性炎症细胞类型的单克隆抗体染色，计数并表达每平方毫米气道壁面积。结果表明，气道阻塞患者气道外膜B淋巴细胞数量明显高于对照组（P < 0.001），粘膜下多形核白细胞数量与吸烟量有关（P < 0.02）。他们还表明BALT在人类中的分布与啮齿动物肺中的分布不同，因为淋巴样聚集在气道外壁而不是粘膜下层。
Lung tissue from 20 patients undergoing resection for a peripheral carcinoma was studied using monoclonal antibodies to identify inflammatory cell types in the peripheral airways and to determine the location of the bronchial-associated lymphoid tissue (BALT). The patients were grouped according to their percent predicted FEV1 (%FEV1) into obstructed (%FEV1 < 80%) and control (%FEV1 > 80%). The resected lungs were filled with dilute cryoembedding media (Tissue-Tek R), frozen over liquid nitrogen, sliced into 2-cm sagittal slices using a band saw, and sampled using a cork bore. Ten serial histologic sections cut from these samples were stained with monoclonal antibodies for specific inflammatory cell types, which were counted and expressed per square millimeter of airway wall area. The results showed that the patients with airway obstruction had more B-lymphocytes in the airway adventitia than did the control subjects (p < 0.001) and that the number of submucosal polymorphonuclear leukocytes is related to the amount smoked (p < 0.02). They also show the BALT has a different distribution in human than in rodent lungs in that the lymphoid collections are found in the outer walls of the airway rather than in the submucosa.