Mitochondrial biogenesis is decreased in skeletal muscle of pig fetuses exposed to maternal high-energy diets
Mitochondrial biogenesis is decreased in skeletal muscle of pig fetuses exposed to maternal high-energy diets
复制标题
暴露于母体高能量饮食的猪胎儿骨骼肌中线粒体生物发生减少
DOI：
10.1017/s1751731116001269
复制
发表时间：
2017
期刊：
影响因子：
3.6
通讯作者：
Chen D. W.
中科院分区：
文献类型：
--
作者：
Zou T. D.;Yu B.;Yu J.;Mao X. B.;Zheng P.;He J.;Huang Z. Q.;He D. T.;Chen D. W.
线粒体在能量稳态调节中发挥着重要作用。此外，线粒体生物发生伴随着骨骼肌发生，我们之前报道过母体高能量饮食抑制了猪胎儿的骨骼肌发生。因此，本研究的目的是评估适度增加母体能量摄入对骨骼肌线粒体生物发生和猪胎儿功能的影响。根据国家研究委员会 (NRC) 的建议，初产纯种大白母猪被分配到正常能量摄入组 (NE) 和高能量摄入组（HE，NRC 建议的 110%）。妊娠第90天，收集胎儿脐静脉血和最长肌（LM）。结果表明，妊娠90天时饲喂HE日粮的母猪增重高于NE日粮母猪（P<0.05）。母体HE饮食增加胎儿脐静脉血清甘油三酯和胰岛素浓度（P<0.05），并有增加稳态模型评估指数的倾向（P=0.08）。此外，HE胎儿LM肌肉中丙二醛浓度升高（P<0.05），抗氧化酶活性（P<0.05）和细胞内NAD+水平降低（P<0.05）。 HE胎儿代谢特征的这些改变伴随着线粒体DNA量的减少(P<0.05)和负责线粒体生物发生和功能的基因的信使RNA表达水平的下调(P<0.05)。我们的结果表明，妊娠期间适度增加能量供应会降低猪胎儿骨骼肌中的线粒体生物发生、功能和抗氧化能力。
Mitochondria plays an important role in the regulation of energy homeostasis. Moreover, mitochondrial biogenesis accompanies skeletal myogenesis, and we previously reported that maternal high-energy diet repressed skeletal myogenesis in pig fetuses. Therefore, the aim of this study was to evaluate the effects of moderately increased maternal energy intake on skeletal muscle mitochondrial biogenesis and function of the pig fetuses. Primiparous purebred Large White sows were allocated to a normal energy intake group (NE) as recommended by the National Research Council (NRC) and a high energy intake group (HE, 110% of NRC recommendations). On day 90 of gestation, fetal umbilical vein blood and longissimus (LM) muscle were collected. Results showed that the weight gain of sows fed HE diet was higher than NE sows on day 90 of gestation (P<0.05). Maternal HE diet increased fetal umbilical vein serum triglyceride and insulin concentrations (P<0.05), and tended to increase the homeostasis model assessment index (P=0.08). Furthermore, HE fetuses exhibited increased malondialdehyde concentration (P<0.05), and decreased activities of antioxidative enzymes (P<0.05) and intracellular NAD+ level (P<0.05) in LM muscle. These alterations in metabolic traits of HE fetuses were accompanied by reduced mitochondrial DNA amount (P<0.05) and down-regulated messenger RNA expression levels of genes responsible for mitochondrial biogenesis and function (P<0.05). Our results suggest that moderately increased energy supply during gestation decreases mitochondrial biogenesis, function and antioxidative capacity in skeletal muscle of pig fetuses.