Intraspecific variation in the Populus balsamifera drought transcriptome
Intraspecific variation in the Populus balsamifera drought transcriptome
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DOI：
10.1111/j.1365-3040.2010.02179.x
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发表时间：
2010-10-01
影响因子：
7.3
通讯作者：
Campbell, Malcolm M.
中科院分区：
文献类型：
--
作者：
Hamanishi, Erin T.;Raj, Sherosha;Campbell, Malcolm M.
干旱是限制杨树生长和生产力的主要因素。杨属树木对抗干旱的能力是基因组对这种环境损害的反应的函数，涉及转录组的重新配置以适当地重塑生长、发育和代谢。在这里，我们测试假设，旨在研究干旱转录组中的种内变异的程度，使用六种不同的杨树。基因型和昂飞基因芯片技术。在一个给定的基因型内，水分亏缺诱导的转录本丰度变化的幅度与该基因型在水分亏缺后保持生长的能力之间存在正相关。具有更相似的干旱响应转录组的基因型也具有更少的基因型差异，如通过微阵列衍生的单特征多态性（SFP）分析所确定的，这表明响应可能在共享更大程度的基因型相似性的个体之间是保守的。这项工作突出了这样一个事实，即一个核心的物种水平的响应可以定义，但是，在杨树干旱响应的基础基因型衍生的复杂性，必须考虑到定义两个物种和属级的响应。
Drought is a major limitation to the growth and productivity of trees in the ecologically and economically important genus Populus. The ability of Populus trees to contend with drought is a function of genome responsiveness to this environmental insult, involving reconfiguration of the transcriptome to appropriately remodel growth, development and metabolism. Here we test hypotheses aimed at examining the extent of intraspecific variation in the drought transcriptome using six different Populus balsamifera L. genotypes and Affymetrix GeneChip technology. Within a given genotype there was a positive correlation between the magnitude of water-deficit induced changes in transcript abundance across the transcriptome, and the capacity of that genotype to maintain growth following water deficit. Genotypes that had more similar drought-responsive transcriptomes also had fewer genotypic differences, as determined by microarray-derived single feature polymorphism (SFP) analysis, suggesting that responses may be conserved across individuals that share a greater degree of genotypic similarity. This work highlights the fact that a core species-level response can be defined; however, the underpinning genotype-derived complexities of the drought response in Populus must be taken into consideration when defining both species- and genus-level responses.