In vivo gene transfer to the mouse eye using an HIV-based lentiviral vector; efficient long-term transduction of corneal endothelium and retinal pigment epithelium
In vivo gene transfer to the mouse eye using an HIV-based lentiviral vector; efficient long-term transduction of corneal endothelium and retinal pigment epithelium
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DOI：
10.1038/sj.gt.3301574
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发表时间：
2001-11-01
期刊：
影响因子：
5.1
通讯作者：
Ali, RR
中科院分区：
文献类型：
--
作者：
Bainbridge, JWB;Stephens, C;Ali, RR
我们已经评估了基于HIV的慢病毒载体在体内局部递送至眼组织的转导概况。视网膜下注射后，绿色荧光蛋白（GFP）报告基因在视网膜色素上皮（RPE）细胞中有效且稳定地表达。有限的转导相邻的光感受器发生在新生小鼠，但在成年动物是低效的。将载体注射到前房中导致角膜内皮细胞的有效且稳定的转导。因此，通过慢病毒载体将基因有效地体内转移到角膜内皮细胞和视网膜色素上皮细胞中可以提供治疗角膜和外层视网膜疾病的有价值的方法。
We have evaluated the transduction profiles of an HIV-based lentiviral vector delivered regionally to ocular tissues in vivo. Following subretinal injection, a green fluorescent protein (GFP) reporter gene was efficiently and stably expressed in retinal pigment epithelial (RPE) cells. Limited transduction of adjacent photoreceptors occurred in newborn mice, but was inefficient in adult animals. Injection of the vector into the anterior chamber resulted in efficient and stable transduction of corneal endothelial cells. Efficient in vivo gene transfer into cells of the corneal endothelium and retinal pigment epithelium by lentiviral vectors may therefore offer a valuable approach to the treatment of disorders of the cornea and outer retina.