Cholesterol-lowering effects of dietary pomegranate extract and inulin in mice fed an obesogenic diet
Cholesterol-lowering effects of dietary pomegranate extract and inulin in mice fed an obesogenic diet
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DOI：
10.1016/j.jnutbio.2017.10.003
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发表时间：
2018-02-01
影响因子：
5.6
通讯作者：
Li, Zhaoping
中科院分区：
文献类型：
--
作者：
Yang, Jieping;Zhang, Song;Li, Zhaoping
背景资料：石榴多酚提取物（Polyphenol-rich石榴extract，PomX）和菊糖多糖（polysaccharide inulin，PomX）均能改善高脂饮食引起的代谢变化，但其具体机制尚不清楚。目的：评价PomX对高脂饮食引起的代谢变化的影响（0.25%）和菊糖（9%）单独或组合对小鼠胆固醇和脂质代谢的影响。雄性C57 BL/6 J小鼠饲喂高脂肪/高蔗糖[HF/HS（32%能量来自脂肪，25%能量来自蔗糖）]饮食，单独或组合补充PomX（0.25%）和菊粉（9%）4周。在干预结束时，血清和肝脏胆固醇，甘油三酯水平，肝基因表达的关键调节胆固醇和脂质代谢，以及粪便胆固醇和胆汁酸排泄determined.Results：膳食补充剂的HF/HS饮食与PomX和菊粉降低肝脏和血清总胆固醇。与单独治疗相比，PomX和菊粉一起补充导致肝脏和血清总胆固醇降低。与HF/HS对照相比，PomX增加了Cyp 7a 1和Cyp 7 b1的基因表达，Cyp 7a 1和Cyp 7 b1是胆汁酸合成途径的关键调节因子。菊粉降低了胆固醇从头合成的关键调节因子Srebf 2和Hmgcr的基因表达，并显着增加了总胆汁酸和中性固醇的粪便消除。与HF/HS对照组相比，仅PomX与菊粉组合显著降低了肝脏和脂质重量。PomX表现出降低肝脏甘油三酯（TG）水平的趋势，而菊糖或PomX-菊糖组合对血清或肝脏TG level.Conclusion没有影响：膳食PomX和菊糖补充剂通过不同的机制降低肝脏和血清总胆固醇，并导致显着增强的降胆固醇效果的组合。(C)2017爱思唯尔公司All rights reserved.
Background: It has been demonstrated in animal studies that both polyphenol-rich pomegranate extract (PomX) and the polysaccharide inulin, ameliorate metabolic changes induced by a high-fat diet, but little is known about the specific mechanisms.Objective: This study evaluated the effect of PomX (0.25%) and inulin (9%) alone or in combination on cholesterol and lipid metabolism in mice.Methods: Male C57BL/6 J mice were fed high-fat/high-sucrose [HF/HS (32% energy from fat, 25% energy from sucrose)] diets supplemented with PomX (0.25%) and inulin (9%) alone or in combination for 4 weeks. At the end of intervention, serum and hepatic cholesterol, triglyceride levels, hepatic gene expression of key regulators of cholesterol and lipid metabolism as well as fecal cholesterol and bile acid excretion were determined.Results: Dietary supplementation of the HF/HS diet with PomX and inulin decreased hepatic and serum total cholesterol. Supplementation with PomX and inulin together resulted in lower hepatic and serum total cholesterol compared to individual treatments. Compared to HF/HS control, PomX increased gene expression of Cyp7a1 and Cyp7b1, key regulators of bile acid synthesis pathways. Inulin decreased gene expression of key regulators of cholesterol de novo synthesis Srebf2 and Hmgcr and significantly increased fecal elimination of total bile acids and neutral sterols. Only PomX in combination with inulin reduced liver and lipid weight significantly compared to the HF/HS control group. PomX showed a trend to decrease liver triglyceride (TG) levels, while inulin or PomX-inulin combination had no effect on either serum or liver TG levels.Conclusion: Dietary PomX and inulin supplementation decreased hepatic and serum total cholesterol by different mechanisms and the combination leading to a significant enhancement of the cholesterol-lowering effect. (C) 2017 Elsevier Inc. All rights reserved.