A structured chitosan-based platform for biomolecule attachment to solid surfaces: Application to DNA microarray preparation
A structured chitosan-based platform for biomolecule attachment to solid surfaces: Application to DNA microarray preparation
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DOI：
10.1021/bc050285a
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发表时间：
2006-03-01
影响因子：
4.7
通讯作者：
De Bellis, G
中科院分区：
文献类型：
--
作者：
Consolandi, C;Severgnini, M;De Bellis, G
提出了一种基于壳聚糖（一种富含胺的多糖）的结构化化学平台，作为一种替代化学方法，用于对固体支持物（在此为载玻片）进行功能化以接枝生物分子。这种方法已被用于制备阵列，使用了两种不同长度（25聚体和70聚体）的氨基修饰寡核苷酸，以用于不同目的。使用这些壳聚糖活化表面的结果表明，寡核苷酸负载能力高，与靶标杂交的可用性好，并且在由DNA聚合酶和DNA连接酶介导的单核苷酸多态性（SNP）检测过程中具有低背景的有效性。通用阵列已被制备并在不同应用中广泛使用，取得了优异的结果。还评估了壳聚糖处理表面在基因表达实验中的性能。
A structured chemical platform based on chitosan, an amine-rich polysaccharide, is presented as an alternative chemistry to functionalize solid support (in this case, glass slides) for grafting biomolecules. This approach has been adopted for generating arrays using amino-modified oligonucleotides with two different lengths (25-mer and 70-mer) for different purposes. Results using these chitosan-activated surfaces indicate high oligonucleotide loading capacity, good availability to hybridization against targets, and effectiveness in enzyme-mediated single nucleotide polymorphism (SNP) detection procedures by DNA polymerase and DNA ligase enzymes with low background. Universal arrays have been prepared and extensively used with excellent results in different applications. The chitosan-treated surfaces were also evaluated for their performance in a gene expression experiment.