BUOYANCY REGULATION IN PHOSPHATE-LIMITED CULTURES OF MICROCYSTIS-AERUGINOSA
BUOYANCY REGULATION IN PHOSPHATE-LIMITED CULTURES OF MICROCYSTIS-AERUGINOSA
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DOI：
10.1111/j.1574-6968.1987.tb02349.x
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发表时间：
1987-06-01
影响因子：
4.2
通讯作者：
MUR, LR
中科院分区：
文献类型：
--
作者：
KONOPKA, A;KROMKAMP, JC;MUR, LR
研究了铜绿微囊藻（Microcystis aeruginosa）在8-16 h光暗循环条件下连续培养时的浮力调节。气泡含量没有系统地变化，在一个范围内的稀释率从0.004至0.015 h-1。辐照度的降低并没有引起气泡含量的显着变化。漂浮细胞的比例在光周期减少，在黑暗期间增加。在三种稀释率下，平行培养物在生长饱和辐照度和较低辐照度下生长。在低辐照度的培养物有较高比例的漂浮细胞和较小的减少浮力在光周期。浮力的损失不是由于气泡的破坏，而是由于重物质的积累。然而，测得的多糖压载物的增加仅占所需压载物的60%。组成镇流器剩余部分的分子未知。在缓解磷酸盐限制后，P限制的培养物在黑暗或光照下孵育时增加了它们的浮力。浮力增加，在黑暗中与多糖含量的减少，而有一个在光中的气泡含量的增加。
Buoyancy regulation was studied in P-limited continuous cultures of Microcystis aeruginosa grown on light-dark cycles of 8-16 h. Gas-vesicle content did not vary systematically over a range of dilution rates from 0.004 to 0.015 h-1. A reduction in irradiance did not cause a significant change in gas-vesicle content. The proportion of floating cells decreased during the photoperiod and increased during the dark period. At three dilution rates, parallel cultures were grown at growth-saturating irradiance and at a lower irradiance. The cultures at low irradiance had a higher proportion of floating cells and a smaller decrease in buoyancy during the light period. The buoyancy losses were not due to destruction of gas vesicles, but, rather, to the accumulation of heavy substances. However, measured increases in polysaccharide ballast accounted for only 60% of the required ballast. The molecule(s) which comprised the remainder of the ballast are unknown. Upon relief of phosphate limitation, P-limited cultures increased their buoyancy when incubated in the dark or light. Buoyancy increases in the dark were correlated with a decrease in polysaccharide content, whereas there was an increase in gas vesicle content in the light.