Targeting of parallel c-myc G-quadruplex by dimeric cyanine dye supramolecular assembly: dependence on the linker length.
Targeting of parallel c-myc G-quadruplex by dimeric cyanine dye supramolecular assembly: dependence on the linker length.
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DOI：
10.1039/c4an01912a
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发表时间：
2015-02
期刊：
影响因子：
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通讯作者：
Lijia Yu;Qianfan Yang;J. Xiang;Hongxia Sun;Li-Xia Wang;Qian Li;Aijiao Guan;Yalin Tang
中科院分区：
文献类型：
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作者：
Lijia Yu;Qianfan Yang;J. Xiang;Hongxia Sun;Li-Xia Wang;Qian Li;Aijiao Guan;Yalin Tang
设计了一系列二聚花青染料来研究它们对平行 c-myc G-四联体的识别以及它们在溶液中的光谱特征。具有不同长度接头的二聚花青染料显示其对双链体 DNA 上的 c-myc 的识别顺序如下：TC-P4 > TC-P5 > TC-P6 > TC-P3 > TC-P7 [数字是接头中重复单元（寡聚氧乙烯）的数量]。这种行为可能是由于它们与 G-四链体的结合所致：二聚体的两个发色团堆叠在 G-四链体的两端，而二聚体的两个发色团之间的连接体协同结合到环和凹槽上。此外，这些染料具有区分宫颈癌细胞 (Hela) 和正常宫颈上皮细胞 (CRL2614) 的能力。这些染料有望成为诊断 G-四链体相关疾病的传感器。
A series of dimeric cyanine dyes were designed to study their recognition for parallel c-myc G-quadruplex as well as their spectral features in solution. Dimeric cyanine dyes having different length linkers show their recognition for c-myc over duplex DNAs following the order: TC-P4 > TC-P5 > TC-P6 > TC-P3 > TC-P7 [the numeral is the number of repeat units (oligo-oxyethylene) in the linker]. This behaviour might result from their binding with G-quadruplex: the two chromophores of dimers stack on both ends of G-quadruplexes while the linker between two chromophores of dimers binds to the loops and grooves cooperatively. Further, these dyes presented an ability to differentiate the cervical cancer cell (Hela) from the normal cervical epithelial cell (CRL2614). These dyes have promising potential to be sensors to diagnose the G-quadruplex related diseases.