Transfer of the movement protein gene between two tobamoviruses: influence on local lesion development.
Transfer of the movement protein gene between two tobamoviruses: influence on local lesion development.
复制标题
两种烟草病毒之间运动蛋白基因的转移：对局部病变发展的影响。
DOI：
10.1016/0042-6822(91)90036-b
复制
发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
Beachy,RN
中科院分区：
文献类型：
--
作者：
Nejidat,A;Cellier,F;Holt,CA;Gafny,R;Eggenberger,AL;Beachy,RN
The effects of transfer of the movement gene between the tobamoviruses tobacco mosaic virus (TMV) and tobacco mild green mosaic virus (TMGMV) were studied. The movement protein (MP) gene of TMGMV was cloned into an infectious cDNA of TMV to build the recombinant virus V23. V23, like TMV and TMGMV, caused systemic infection inNicotiana tabacumXanthi. InN. sylvestrisV23 and TMV spread systemically although TMGMV produces necrotic local lesions on this host. V23 and TMV cause systemic infection on tomato plants while TMGMV does not infect tomato. In Xanthi nc plants, V23 produced necrotic local lesions similar in size to those produced by TMGMV. On the other hand in transgenic Xanthi nc tobacco plants that express a gene encoding the MP of TMV the necrotic lesions produced by V23 and TMGMV were similar in size to those produced by TMV. These results indicate that the size of necrotic lesions produced by TMGMV and TMV on Xanthi nc plants is influenced by the MP gene.
