AGROBACTERIUM-TUMEFACIENS-MEDIATED TRANSFORMATION OF SAFFLOWER (CARTHAMUS-TINCTORIUS L) CV CENTENNIAL
AGROBACTERIUM-TUMEFACIENS-MEDIATED TRANSFORMATION OF SAFFLOWER (CARTHAMUS-TINCTORIUS L) CV CENTENNIAL
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DOI：
10.1007/bf00233097
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发表时间：
1992-10-01
影响因子：
6.2
通讯作者：
BERGMAN, JW
中科院分区：
文献类型：
--
作者：
YING, MC;DYER, WE;BERGMAN, JW
Efficient callus formation was achieved from cotyledon, stem, and leaf explants of the domestic safflower cultivar 'Centennial' on MS salts medium containing 1 mg/L BAP and 1 mg/L NAA. Shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. 'Centennial' explants inoculated with Agrobacterium tumefaciens containing NPT II and GUS genes produced kanamycin-resistant calli from which buds were regenerated. Transformation and stable integration of transgenes was confirmed by GUS assay and DNA hybridization in kanamycin-resistant calli, and GUS assay in regenerated shoots.
