Two-electron reduction of cytochrome c oxidase triggers a conformational transition.
Two-electron reduction of cytochrome c oxidase triggers a conformational transition.
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细胞色素 c 氧化酶的双电子还原触发构象转变。
DOI：
10.1016/0014-5793(86)81197-8
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发表时间：
1986
期刊：
影响因子：
3.5
通讯作者：
Malmström,BG
中科院分区：
文献类型：
--
作者：
Scholes,CP;Malmström,BG
氰化物诱导的光变化在437 nm以下的快速氰化物抑制细胞色素氧化酶的缓慢增加已被作为一个功能的电子捐赠从ferrocytochromecto细胞色素和铜。光学变化的初始速率是这个数的抛物线函数。结果进行了分析，在一个模型中，除了电子引起的构象转变，允许快速氰化物结合。结合之后是一个缓慢的分子内反应，负责光学变化。分析表明，只有细胞色素和Cureduced的分子才能发生构象变化，这可能与氧化酶的质子泵机制有关。
The slow increase of a cyanide-induced optical change at 437 nm following rapid cyanide inhibition of cytochrome oxidase has been followed as a function of the number of electrons donated from ferrocytochromecto cytochromeaand Cua. The initial rate of optical change is a parabolic function of this number. The results have been analyzed in terms of a model where addition of electrons causes a conformational transition allowing rapid cyanide binding. The binding is followed by a slow intramolecular reaction responsible for the optical change. The analysis demonstrates that only molecules with both cytochromeaand Cuareduced can undergo the conformational change, which is suggested to be involved in the proton-pump mechanism of the oxidase.