Direct evidence that the poly(A) tail of influenza A virus mRNA is synthesized by reiterative copying of a U track in the virion RNA template
Direct evidence that the poly(A) tail of influenza A virus mRNA is synthesized by reiterative copying of a U track in the virion RNA template
复制标题
DOI：
10.1128/jvi.73.4.3473-3476.1999
复制
发表时间：
1999-04-01
影响因子：
5.4
通讯作者：
Brownlee, GG
中科院分区：
文献类型：
--
作者：
Poon, LLM;Pritlove, DC;Brownlee, GG
流感病毒mRNA的聚(A)尾被认为是通过重复复制病毒RNA模板5‘端附近的U轨道而合成的。这一假设已被广泛接受，但到目前为止，还没有直接的实验证据支持这一假设。在这里，我们报告了这样的直接证据，基于以下事实：(I)在体外和体内，用A轨道取代U轨道可以指导用聚(U)尾巴合成产物；(Ii)在体外，阻断U轨道会取消多聚腺苷基化。
The poly(A) tail of influenza virus mRNA is thought to be synthesized by reiterative copying of the U track near the 5' end of the virion RNA template. This has been widely accepted as a plausible hypothesis, but until now there has been no direct experimental evidence for it. Here, we report such direct evidence based on the fact that (i) replacing the U track with an A track directs synthesis of products with poly(U) tails, both in vitro and in vivo, and (ii) interrupting the U track abolishes polyadenylation in vitro.