Cloning and functional characterization of the Rhizopus oryzae high affinity iron permease (rFTR1) gene
Cloning and functional characterization of the Rhizopus oryzae high affinity iron permease (rFTR1) gene
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DOI：
10.1016/j.femsle.2004.04.031
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发表时间：
2004-06-01
影响因子：
2.1
通讯作者：
Ibrahim, AS
中科院分区：
文献类型：
--
作者：
Fu, Y;Lee, H;Ibrahim, AS
毛霉根霉是毛霉病最常见的病原菌。临床和动物模型数据清楚地表明，血清铁水平升高易使宿主发生毛霉菌病。因此，高亲和性铁通透酶（rFTR 1）编码的蛋白质需要从环境中吸收铁，很可能是一个关键的决定因素，毒力的R。米。我们已经克隆rFTR 1通过使用PCR方法依赖于简并引物设计的保守区域的酿酒酵母高亲和力铁通透酶。对2.0 kb EcoRI基因组克隆的序列分析显示，缺失内含子的1107 bp的单个开放阅读框。推测的rFtr1p与已知的来自白色念珠菌（Candida albicans）和S.酿酒酵母（44%同一性）。in R.此外，rFTR 1在缺铁培养基中表达，而在富铁培养基中不表达。最后，rFTR 1恢复了S.酿酒酵母能在铁限制培养基上生长并摄取放射性标记的铁，而S.用空载体转化的酿酒酵母则没有。这些数据表明，我们已经克隆了编码一个R。高亲和力的铁通透酶和推定的rFtr1p参与从铁缺乏环境中同化铁。(C)2004年，欧洲微生物学会联合会。Elsevier B.V.出版，保留所有权利。
Rhizopus oryzae is the most common etiologic agent of mucormycosis. Clinical and animal model data clearly demonstrate that the presence of elevated available serum iron predisposes the host to develop mucormycosis. Therefore, the high affinity iron permease (rFTR1) which encodes a protein required to scavenge iron from the environment, is highly likely to be a critical determinant of virulence for R. oryzae. We have cloned rFTR1 by using a PCR approach relying on degenerate primers designed from the conserved regions of Saccharomyces cerevisiae high affinity iron permease. Sequence analysis of a 2.0 kb EcoRI genomic clone revealed a single open reading frame of 1107 bp that lacked introns. The putative rFtr1p had significant homology to known fungal high affinity iron permeases from Candida albicans (46% identity) and S. cerevisiae (44% identity). In R. oryzae, rFTR1 was expressed in iron-depleted and not in iron-rich media. Finally, rFTR1 restored the ability of an ftr1 null mutant of S. cerevisiae to grow on iron-limited medium and to take up radiolabeled iron, whereas S. cerevisiae transformed with the empty vector did not. These data demonstrate that we have cloned the gene encoding a R. oryzae high affinity iron permease and the putative rFtr1p is involved in assimilation of iron from iron-depleted environments. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.