The preparation and antioxidant activity of glucosamine sulfate
The preparation and antioxidant activity of glucosamine sulfate
复制标题
DOI：
10.1007/s00343-009-9135-x
复制
发表时间：
2009-07
影响因子：
--
通讯作者：
R. Xing;Song Liu;Lin Wang;Shengbao Cai;Huahua Yu;Jinhua Feng;Pengcheng Li
中科院分区：
文献类型：
--
作者：
R. Xing;Song Liu;Lin Wang;Shengbao Cai;Huahua Yu;Jinhua Feng;Pengcheng Li
以甲壳素酸水解生成的盐酸氨基葡萄糖为原料，采用离子交换法制备硫酸氨基葡萄糖。旋光度和元素分析表征了其纯度。此外，在各种已建立的体外系统中研究了壳聚糖衍生物-氨基葡萄糖硫酸盐的抗氧化效力，例如超氧化物（O2−）/羟基（·OH）自由基清除、还原力、铁离子螯合。得到以下结果：首先，硫酸氨基葡萄糖对超氧自由基有明显的清除作用。例如，0.8 mg/mL 的硫酸氨基葡萄糖的 O2-清除活性为 92.11%。其次，硫酸氨基葡萄糖的·OH清除活性也很强，在3.2 mg/mL时约为50%。第三，硫酸氨基葡萄糖的还原能力更明显。硫酸氨基葡萄糖的还原力在 0.75 mg/mL 时为 0.643。然而，其对亚铁离子的螯合效力较弱。此外，除亚铁离子螯合力外，氨基葡萄糖硫酸盐的自由基清除率和还原力均呈浓度依赖性，并随浓度的增加而增加，但其亚铁离子螯合力则随着浓度的增加而下降。硫酸氨基葡萄糖的多种抗氧化活性表现为还原力和超氧化物/羟基自由基清除能力。这些体外结果表明硫酸氨基葡萄糖可以有效地用作健康或功能食品的成分，以减轻氧化应激。
Glucosamine sulfate was prepared from glucosamine hydrochloride that was produced by acidic hydrolysis of chitin by ion-exchange method. Optical rotation and elemental analysis characterized the degree of its purity. In addition, the antioxidant potency of chitosan derivative-glucosamine sulfate was investigated in various establishedin vitrosystems, such as superoxide (O2−)/hydroxyl (·OH) radicals scavenging, reducing power, iron ion chelating. The following results are obtained: first, glucosamine sulfate had pronounced scavenging effect on superoxide radical. For example the O2−scavenging activity of glucosamine sulfate was 92.11% at 0.8 mg/mL. Second, the ·OH scavenging activity of glucosamine sulfate was also strong, and was about 50% at 3.2 mg/mL. Third, the reducing power of glucosamine sulfate was more pronounced. The reducing power of glucosamine sulfate was 0.643 at 0.75 mg/mL. However, its potency for ferrous ion chelating was weak. Furthermore, except for ferrous ion chelating potency, the scavenging rate of radical and reducing power of glucosamine sulfate were concentration-dependent and increased with their increasing concentrations, but its ferrous ion chelating potency decreased with the increasing concentration. The multiple antioxidant activities of glucosamine sulfate were evidents of reducing power and superoxide/hydroxyl radicals scavenging ability. Thesein vitroresults suggest the possibility that glucosamine sulfate could be used effectively as an ingredient in health or functional food, to alleviate oxidative stress.