Enteric bacteria may play a role in mammalian arsenic metabolism
Enteric bacteria may play a role in mammalian arsenic metabolism
复制标题
肠道细菌可能在哺乳动物砷代谢中发挥作用
DOI：
--
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
G. Endo
中科院分区：
文献类型：
--
作者：
K. Kuroda;Kaoru Yoshida;A. Yasukawa;H. Wanibuchi;S. Fukushima;G. Endo
通过饮用水给予二甲基胂酸6个月的大鼠的盲肠内容物在含有10 mg l-1二甲基胂酸的GAM培养基中培养。 砷化合物的文化进行了分析，通过离子色谱与电感耦合等离子体质谱（IC-ICP-MS）。二甲胂酸被代谢。从培养物中分离出两株大肠杆菌菌株A3-4和A3-6。这些菌株代谢二甲基胂酸，并产生两个身份不明的砷化合物，M-2和M-3。A3-6甲基化二甲基胂酸为三甲基氧化胂。这两种菌株代谢氧化三甲基胂，并产生一种身份不明的砷化合物，M-1。这些未知的砷化合物与在服用二甲基胂酸的大鼠的尿液和粪便中检测到的化合物相同。菌株能有效地将砷酸盐还原为亚砷酸盐。这些细菌代谢二甲基胂酸需要半胱氨酸，但不需要谷胱甘肽。这些结果表明，肠道细菌具有不同于哺乳动物的砷代谢，它们可能在哺乳动物的砷代谢中发挥作用。版权所有© 2001约翰威利父子有限公司。
The cecal content of rats administered dimethylarsinic acid for 6 months via drinking water was cultured in GAM medium with 10 mg l−1 of dimethylarsinic acid. Arsenic compounds in the culture were analyzed by ion chromatography with inductively coupled plasma mass spectrometry (IC–ICP-MS). Dimethylarsinic acid was metabolized. Two bacterial Escherichia coli strains, A3-4 and A3-6, were isolated from the culture. These strains metabolized dimethylarsinic acid and yielded two unidentified arsenic compounds, M-2 and M-3. A3-6 methylated dimethylarsinic acid to trimethylarsine oxide. Both strains metabolized trimethylarsine oxide and yielded an unidentified arsenic compound, M-1. These unknown arsenic compounds were the same compounds as detected in the urine and the feces of rats administered dimethylarsinic acid. The strains reduced arsenate to arsenite efficiently. Cysteine was required for metabolism of dimethylarsinic acid by these bacteria, but glutathione was not required. These results strongly suggested that the intestinal bacteria have a different arsenic metabolism from that in mammals and that they may play a possible role in mammalian arsenic metabolism. Copyright © 2001 John Wiley & Sons, Ltd.
DOI：
10.1006/taap.1998.8409
发表时间：
1998
期刊：
Toxicology and applied pharmacology.
影响因子：
--
作者：
Wildfang,E;Zakharyan,RA;Aposhian,HV
通讯作者：
Aposhian,HV