Nuclear magnetic resonance spectroscopy and imaging in animal research.
Nuclear magnetic resonance spectroscopy and imaging in animal research.
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DOI：
10.1093/ilar.42.3.189
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发表时间：
2001
期刊：
影响因子：
2.5
通讯作者：
J. Chatham;S. Blackband
中科院分区：
文献类型：
--
作者：
J. Chatham;S. Blackband
核磁共振（NMR）波谱和成像可用于无创地研究各种系统中的生物过程，如蛋白质溶液、单细胞、分离的灌注器官和体内组织。也可以结合不同的核磁共振技术，在同一实验中获得代谢、解剖和生理信息。本文简要介绍了核磁共振的基本原理，并概述了核磁共振波谱和成像的优缺点。提出了一些核磁共振波谱和成像的潜在应用实例，展示了使用这些技术可以提出的问题范围。讨论了在生物医学研究项目中使用核磁共振技术对特定研究所需动物总数的潜在影响，以及研究中使用的动物数量。文章总结了个人对核磁共振技术持续改进对未来动物研究应用的影响的看法。
Nuclear magnetic resonance (NMR) spectroscopy and imaging can be used to investigate, noninvasively, a wide range of biological processes in systems as diverse as protein solutions, single cells, isolated perfused organs, and tissues in vivo. It is also possible to combine different NMR techniques enabling metabolic, anatomical, and physiological information to be obtained in the same experiment. This review provides a simple overview of the basic principles of NMR and outlines both the advantages and disadvantages of NMR spectroscopy and imaging. A few examples of potential applications of NMR spectroscopy and imaging are presented, which demonstrate the range of questions that can be asked using these techniques. The potential impact of using NMR techniques in a biomedical research program on the total number of animals required for specific investigations, as well as the number of animals used in research, are discussed. The article concludes with a personal perspective on the impact of continuing improvements in NMR technology for future applications in animal research.