Ratio-based decisions and the quantitative analysis of cDNA microarray images.
Ratio-based decisions and the quantitative analysis of cDNA microarray images.
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DOI：
10.1117/12.281504
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发表时间：
1997-10-01
影响因子：
3.5
通讯作者：
Bittner, M L
中科院分区：
文献类型：
--
作者：
Chen, Y;Dougherty, E R;Bittner, M L
基因表达可以通过荧光标记的mRNA与cDNA微阵列上的靶杂交来定量分析。通过取单个基因的平均表达水平的比率来实现来自共杂交样品的基因表达水平的比较。一种新的图像分割方法，以确定cDNA靶位点和假设检验和置信区间的发展，以量化的显着性观察到的差异表达率。特别是，概率密度的比率和最大似然估计的分布推导，和信号校准的迭代过程。
Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed.