A Characterization of the DNA Data Storage Channel
A Characterization of the DNA Data Storage Channel
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DOI：
10.1038/s41598-019-45832-6
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发表时间：
2019-07-04
影响因子：
4.6
通讯作者：
Grass, Robert N.
中科院分区：
文献类型：
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作者：
Heckel, Reinhard;Mikutis, Gediminas;Grass, Robert N.
由于其寿命长和巨大的信息密度，DNA，编码生物信息的分子，已成为一个有前途的档案存储介质。然而，由于技术的限制，数据只能被写入到许多以无序方式存储的短DNA分子上，并且只能通过从这个DNA池中采样来读取。此外，DNA的写入（合成）、阅读（测序）、储存和处理，特别是通过PCR的扩增中的缺陷导致DNA分子的损失并在分子内引起错误。为了设计DNA存储系统，对错误和分子损失的定性和定量理解至关重要。在本文中，我们通过分析我们自己的实验以及从两个不同的组的实验数据，这些错误概率的特征。我们发现，分子内的错误主要是由于合成和测序，而在处理和存储的缺陷导致序列的显着损失。我们研究的目的是通过提供对DNA数据存储通道的定量和定性理解来帮助指导未来DNA数据存储系统的设计。
Owing to its longevity and enormous information density, DNA, the molecule encoding biological information, has emerged as a promising archival storage medium. However, due to technological constraints, data can only be written onto many short DNA molecules that are stored in an unordered way, and can only be read by sampling from this DNA pool. Moreover, imperfections in writing (synthesis), reading (sequencing), storage, and handling of the DNA, in particular amplification via PCR, lead to a loss of DNA molecules and induce errors within the molecules. In order to design DNA storage systems, a qualitative and quantitative understanding of the errors and the loss of molecules is crucial. In this paper, we characterize those error probabilities by analyzing data from our own experiments as well as from experiments of two different groups. We find that errors within molecules are mainly due to synthesis and sequencing, while imperfections in handling and storage lead to a significant loss of sequences. The aim of our study is to help guide the design of future DNA data storage systems by providing a quantitative and qualitative understanding of the DNA data storage channel.