ULS: a versatile method of labeling nucleic acids for FISH based on a monofunctional reaction of cisplatin derivatives with guanine moieties
ULS: a versatile method of labeling nucleic acids for FISH based on a monofunctional reaction of cisplatin derivatives with guanine moieties
复制标题
DOI：
10.1159/000015390
复制
发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
Tanke, HJ
中科院分区：
文献类型：
--
作者：
Wiegant, JCAG;van Gijlswijk, RPM;Tanke, HJ
广泛扩展现有的化学DNA标记技术的分子细胞遗传学的描述。称为通用连接系统（ULS（TM）），它是基于单反应性顺铂衍生物在DNA中鸟嘌呤部分的N7位置反应的能力。简单的重复探针，cosmetry，PAC，和染色体特异性的绘画探针标记的ULS和用于一系列的荧光原位杂交实验间期和中期细胞。它表明，ULS标记的探针，在一般情况下，执行以及更传统的酶标记的探针。与酶标记技术相比，ULS标记的优点在于它是一种快速且简单的方法，并且标记可以容易地扩大用于批量探针合成。此外，利用ULS标记，可以标记降解的DNA，已知酶标记的情况不能令人满意地进行。版权所有（C）1999 S. Karger AG，巴塞尔。
The broad extension of an existing chemical DNA labeling technique for molecular cytogenetics is described. Called the Universal Linkage System (ULS(TM)), it is based on the capability of monoreactive cisplatin derivatives to react at the N7 position of guanine moieties in DNA. Simple repetitive probes, cosmids, PACs, and chromosome-specific painting probes were labeled by ULS and used in a series of multicolor fluorescence in situ hybridization experiments on interphase and metaphase cells. It is demonstrated that ULS-labeled probes, in general, perform as well as the more conventional enzymatically labeled probes. The advantage of ULS labeling over enzymatic labeling techniques is that it is a fast and simple procedure, and that the labeling can easily be scaled up for bulk probe synthesis. In addition, with ULS labeling it is possible to label degraded DNA, a situation in which enzymatic labeling is known to perform unsatisfactorily. Copyright (C) 1999 S. Karger AG, Basel.