Periodontal Ligament Stem Cells Regulate Apoptosis of Neutrophils.
Periodontal Ligament Stem Cells Regulate Apoptosis of Neutrophils.
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DOI：
10.1515/med-2017-0004
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Wang Q;Ding G;Xu X
牙周膜干细胞(PDLSCs)是牙周炎细胞治疗和生物根再生的理想细胞来源。在这项研究中，我们研究了PDLSCs对中性粒细胞的影响，中性粒细胞是天然免疫的关键成分，并探讨了其潜在机制。在细胞接触培养和Transwell培养条件下，检测PDLSCs对静止期中性粒细胞和IL-8激活的中性粒细胞增殖和凋亡的影响。我们发现，无论在细胞接触还是Transwell培养条件下，PDLSCs都能促进中性粒细胞的增殖，减少中性粒细胞的凋亡。抗IL-6抗体可降低PDLSCs对中性粒细胞凋亡的抑制作用。IL-6浓度为10 ng/ml和20 ng/ml时，对中性粒细胞的凋亡有明显的抑制作用。总之，PDLSCs可以通过IL-6减少中性粒细胞的凋亡。
Periodontal ligament stem cells (PDLSCs) are promising cell resource for the cell-based therapy for periodontitis and regeneration of bio-root. In this study, we investigated the effect of PDLSCs on neutrophil, a critical constituent of innate immunity, and the underlying mechanisms. The effect of PDLSCs on the proliferation and apoptosis of resting neutrophils and IL-8 activated neutrophils was tested under cell-cell contact culture and Transwell culture, with or without anti-IL-6 neutralizing antibody. We found that PDLSCs could promote the proliferation and reduce the apoptosis of neutrophils whether under cell-cell contact or Transwell culture. Anti-IL-6 antibody reduced PDLSCs-mediated inhibition of neutrophil apoptosis. IL-6 at the concentration of 10ng/ml and 20ng/ml could inhibit neutrophil apoptosis statistically. Collectively, PDLSCs could reduce the apoptosis of neutrophils via IL-6.