Developmental analysis of ceruloplasmin gene and liver formation in zebrafish
Developmental analysis of ceruloplasmin gene and liver formation in zebrafish
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DOI：
10.1016/s0925-4773(01)00330-6
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发表时间：
2001-05-01
影响因子：
2.6
通讯作者：
Korzh, V
中科院分区：
文献类型：
--
作者：
Korzh, S;Emelyanov, A;Korzh, V
以血浆铜蓝蛋白（Cp）为特异性标记物，研究了斑马鱼正常胚胎发育过程中肝脏的形成。Cp的不对称表达在16 hpf的背内胚层中，随后在卵黄囊的早期肝细胞中被检测到。32 hpf后可检测到肝原基。在oep（-/-）突变体中，缺乏背内胚层，肝脏不能形成。在无脊索的flh（-/-）突变体中，肝脏的不对称性已经丧失。因此，脊索、背侧内胚层和卵黄囊内胚层在斑马鱼肝脏形成中起作用。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Formation of the liver in zebrafish has been analyzed during normal embryogenesis using ceruloplasmin (Cp) as a specific marker. The asymmetric expression of Cp has been detected in dorsal endoderm at 16 hpf and later in the early hepatic cells in the yolk sac. The liver primordium can be detected after 32 hpf. In oep(-/-) mutant, which lacks dorsal endoderm, the liver fails to form. In the notochordless flh(-/-) mutant, the asymmetry of the liver has been lost. Therefore the notochord, dorsal endoderm and endoderm of the yolk sac play a role in liver formation in zebrafish. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.