Production of Anthocyanins by Vitis Cells in Suspension Culture
Production of Anthocyanins by Vitis Cells in Suspension Culture
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DOI：
10.1271/bbb1961.47.2185
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
T. Yamakawa;S. Kato;K. Ishida;T. Kodama;Y. Minoda
中科院分区：
文献类型：
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作者：
T. Yamakawa;S. Kato;K. Ishida;T. Kodama;Y. Minoda
在没有光照的情况下，葡萄杂交种的愈伤组织中积累了大量的花青素。对悬浮培养的葡萄细胞生产花青素的培养条件进行了研究。高浓度的蔗糖和低浓度的磷酸盐会显着增加花青素的形成，而高浓度的硝酸盐、磷酸盐和2, 4-D则抑制色素的形成。这些营养素的作用取决于共存营养素的浓度。通气速率的调节对于浸没通气培养物中花青素的形成很重要，光照射增强了培养细胞中花青素的形成。
A large amount of anthocyanin was accumulated in the callus tissues of Vitis hybrids without light irradiation.Culture conditions for the production of anthocyanins by Vitis cells in suspension cultures were investigated. High sucrose and low phosphate concentrations brought about a marked increase of anthocyanin formation, while high concentrations of nitrate, phosphate, and 2, 4-D repressed the pigment formation. The effects of these nutrients depended on the concentrations of coexistent ones.Regulation of the aeration rate was important for anthocyanin formation in submerged aerated cultures and light irradiation enhanced anthocyanin formation in cultured cells.