Raman imaging of lipid bilayer membrane by surface enhanced Raman scattering, SPIE Biomedical Vibrational Spectroscopy
Raman imaging of lipid bilayer membrane by surface enhanced Raman scattering, SPIE Biomedical Vibrational Spectroscopy
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通过表面增强拉曼散射、SPIE 生物医学振动光谱对脂质双层膜进行拉曼成像
DOI：
10.1117/12.2286839
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Saito Y
中科院分区：
文献类型：
--
作者：
Mori M;Abe S;Kondo T;Saito Y
我们利用表面增强拉曼光谱（Sers）研究了二维脂质双层的光谱成像。在玻璃基质上孵育的DSPC脂质双层涂覆一层薄银。由于银膜的强电磁增强和对脂质分子的亲和力，在0.1 mW的入射激光功率下，在1 s的曝光时间内获得了单个双层的拉曼光谱。在C-H振动区的光谱，这是敏感的双层配置，随机堆叠区占主导地位的CH 3不对称拉伸模式，而平坦的地区，包括双双层显示典型的Sers光谱。随机堆叠区域的光谱特征被解释为许多游离脂质分子的存在，这是由成对DSPC分子的DFT计算支持。我们的方法可用于揭示单个脂质双层的局部结晶度，这是传统拉曼成像难以评估的。
We investigated two-dimensional lipid bilayers by spectroscopic imaging with surface enhanced Raman spectroscopy (SERS). A DSPC lipid bilayer incubated on a glass substrate was coated with a thin layer of silver. Due to the strong electromagnetic enhancement of the silver film and the affinity to lipid molecules, the Raman spectrum of a single bilayer was obtained in a 1 s exposure time with 0.1 mW of incident laser power. In the C-H vibrational region of the spectra, which is sensitive to bilayer configurations, a randomly stacked area was dominated by the CH3 asymmetric-stretch mode, whereas flat areas including double bilayers showed typical SERS spectra. The spectral features of the randomly stacked area are explained by the existence of many free lipid molecules, which is supported by DFT calculations of paired DSPC molecules. Our method can be applied to reveal the local crystallinity of single lipid bilayers, which is difficult to assess by conventional Raman imaging.