Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity
Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity
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脑内皮细胞的分离和表征：形态学和酶活性
DOI：
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发表时间：
1980
影响因子：
4.7
通讯作者：
M. Bitensky
中科院分区：
文献类型：
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作者：
Stuart K. Williams;John F. Gillis;M. A. Matthews;Roger C. Wagnert;M. Bitensky
Microvessels were isolated from rat brain using a double collagenase treatment which removed the endothelial basement membranes. The isolate was characterized by intact luminal and abluminal membranes and an absence of pericytes and astrocyte membranes. Minimal contamination by 5′‐nucleotidase, an enzyme believed exclusively localized within the plasma membranes of neuroglia, established the purity of the isolated microvessels. Enrichment of alkaline phosphatase and ‐γ‐glutamyl transpeptidase activity in microvessel preparations supports the endothelial localization of these enzymes.
