Unified quantitative characterization of epithelial tissue development
Unified quantitative characterization of epithelial tissue development
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DOI：
10.7554/elife.08519
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发表时间：
2015-12-12
期刊：
影响因子：
7.7
通讯作者：
Bellaiche, Yohanns
中科院分区：
文献类型：
--
作者：
Guirao, Boris;Rigaud, Stephane U.;Bellaiche, Yohanns
了解调节发育的机制需要对细胞分裂、重排、细胞大小和形状变化以及细胞分裂进行定量表征。我们开发了一个多尺度的形式主义，涉及到每个细胞过程的组织生长和形态发生的特征。在计算机模拟上验证了形式主义，我们分别量化了果蝇背胸和翅蛹上皮细胞中的所有形态发生事件，以获得全面的统计图，将细胞和组织尺度动态联系起来。虽然在全球范围内细胞形状的变化，重排和分裂都显着参与组织形态发生，局部，它们的相对参与显示在空间和时间上的主要变化。通过阻断分裂，我们分析了分裂对重排、细胞形状变化和组织形态发生的影响。最后，通过将形式主义与机械应力测量相结合，我们证明了组织伸长，细胞分裂和应力模式之间的意外相互作用。我们的形式主义提供了一个新的和严格的方法来揭示组织发育的机制。
Understanding the mechanisms regulating development requires a quantitative characterization of cell divisions, rearrangements, cell size and shape changes, and apoptoses. We developed a multiscale formalism that relates the characterizations of each cell process to tissue growth and morphogenesis. Having validated the formalism on computer simulations, we quantified separately all morphogenetic events in the Drosophila dorsal thorax and wing pupal epithelia to obtain comprehensive statistical maps linking cell and tissue scale dynamics. While globally cell shape changes, rearrangements and divisions all significantly participate in tissue morphogenesis, locally, their relative participations display major variations in space and time. By blocking division we analyzed the impact of division on rearrangements, cell shape changes and tissue morphogenesis. Finally, by combining the formalism with mechanical stress measurement, we evidenced unexpected interplays between patterns of tissue elongation, cell division and stress. Our formalism provides a novel and rigorous approach to uncover mechanisms governing tissue development.