Influence of Mesenchymal Stem Cells with Endothelial Progenitor Cells in Co-culture on Osteogenesis and Angiogenesis: An In Vitro Study
Influence of Mesenchymal Stem Cells with Endothelial Progenitor Cells in Co-culture on Osteogenesis and Angiogenesis: An In Vitro Study
复制标题
间充质干细胞与内皮祖细胞共培养对成骨和血管生成的影响：体外研究
DOI：
10.1016/j.arcmed.2013.09.009
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发表时间：
2013-10-01
影响因子：
7.7
通讯作者：
Wang, Zuolin
中科院分区：
文献类型：
--
作者：
Li, Qiong;Wang, Zuolin
背景和目标。骨骼是一种高度血管化的组织，依赖于血管和骨细胞之间紧密的空间和时间连接来维持骨骼的完整性。考虑到成骨和血管生成之间的复杂联系，间充质干细胞（MSC）和内皮祖细胞（EPC）之间的通讯是协调骨形成的最重要的细胞相互作用之一，这并不奇怪。本研究的目的是评估共培养的骨髓源性内皮祖细胞（BM-EPC）和间充质干细胞（MSC）在体外血管生成和成骨中的相互作用。方法。采用密度梯度离心结合贴壁法从犬髂嵴骨髓中分离MSCs和BM-EPCs，并通过表面标志物、细胞增殖和免疫细胞化学进行体外鉴定。我们在共培养系统中使用 MSC 和 BM-EPC 的直接接触方法。使用碱性磷酸酶 (ALP) 活性测定、基质矿化测定、Matrigel 2D 测定和基因表达来检查共培养细胞和非共培养细胞。结果。第3天后，与单独的MSC相比，共培养的细胞中的ALP活性和结节钙化显着增加，并且共培养细胞的成管活性也高于单独的BM-EPC。骨和血管生成标志物的表达增强，超过了单独培养的 MSC 和 BM-EPC 的表达水平。结论。 BM-EPCs与MSCs共培养可以促进成骨和血管生成。这种共培养系统可广泛用于改造各种其他组织类型。 (C) 2013 年IMSS。由爱思唯尔公司出版
Background and Aims. Bone is a highly vascularized tissue reliant on the close spatial and temporal connection between blood vessels and bone cells to maintain skeletal integrity. Considering the intricate connection between osteogenesis and angiogenesis, it is not surprising that communication between mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) is one of the most important cellular interactions that orchestrates bone formation. The aim of this study was to evaluate the interaction of co-cultured bone marrow-derived endothelial progenitor cells (BM-EPCs) and mesenchymal stem cells (MSCs) in angiogenesis and osteogenesis in vitro.Methods. MSCs and BM-EPCs were isolated from bone marrow of dogs' iliac crest using density gradient centrifugation combined with adhesion method and identified with surface markers, cell proliferation and immunocytochemistry in vitro. We used the direct contact method of MSCs and BM-EPCs in a co-culture system. Co-cultured cells and non-co-cultured cells were examined using the alkaline phosphatase (ALP) activity assay, matrix mineralization assay, Matrigel 2D assay and gene expression.Results. ALP activity and calcification of nodules significantly increased in the co-cultured cells compared with MSCs alone after day 3, and tubulogenic activity of the co-cultured cells was also higher than BM-EPCs alone. Expression of bone and angiogenic markers were enhanced beyond expression levels of MSCs and BM-EPCs cultured alone.Conclusions. BM-EPCs co-cultured with MSCs can promote osteogenesis and angiogenesis. This co-cultured system may be broadly useful in engineering a variety of other tissue types. (C) 2013 IMSS. Published by Elsevier Inc.