Functional analysis of transcriptional repressor Otx3/Dmbx1
Functional analysis of transcriptional repressor Otx3/Dmbx1
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DOI：
10.1016/j.febslet.2005.04.042
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发表时间：
2005-05-23
期刊：
影响因子：
3.5
通讯作者：
Seino, S
中科院分区：
文献类型：
--
作者：
Kimura, K;Miki, T;Seino, S
Otx3/Dmbx1是配对类同源域转录因子的成员。在本研究中，我们发现Otx3/Dmbx1通过在体外P3C （TAATCCGATTA）序列上与Otx2形成异源二聚体来抑制Otx2介导的转激活。Otx3/Dmbx1的156个氨基酸区（残基1-156）是其抑制因子活性所必需的，并直接与Otx2相互作用。原位杂交证实Otx3/Dmbx1和Otx2在脑切片中共定位。这些数据表明，Otx3/Dmbx1在发育中的大脑中抑制otx2介导的转录。我们还确定了Otx3/Dmbx1的一致结合序列[TAATCCGATTA和TAATCC(N24)TAATCC]。(c) 2005年欧洲生化学会联合会。Elsevier B.V.版权所有。
Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1-156) of Otx3/Dmbx1 is required for its repressor activity, and interacts directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain sections was confirmed by in situ hybridization. These data suggest that Otx3/Dmbx1 represses Otx2-mediated transcription in the developing brain. We also identified the consensus binding sequence [TAATCCGATTA and TAATCC(N24)TAATCC] of Otx3/Dmbx1. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.