Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay.
Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay.
复制标题
条带测定中 CXCL12 处理的迁移神经祖细胞的活细胞成像和 Rac1-GTP 水平。
DOI：
10.1016/j.dib.2015.09.048
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发表时间：
2015
期刊：
影响因子：
1.2
通讯作者：
Zheng,JialinC
中科院分区：
文献类型：
--
作者：
Zhang,Min;Song,Aihong;Lai,Siqiang;Qiu,Lisha;Huang,Yunlong;Chen,Qiang;Zhu,Bing;Xu,Dongsheng;Zheng,JialinC
本数据文章包含与Zhang et al.（2015）[1]题为“Applications of Stripe Assay in the Study of CXCL 12-mediated Neural Progenitor Cell Migration and Polarization”的研究文章相关的三张图和三个视频，该研究文章使用stripe Assay研究小鼠神经祖细胞（NPC）迁移和极化。目前的文章描述了用于培养NPC的神经球方法。采用免疫细胞化学方法对神经球和单层中的NPC进行表征，所述免疫细胞化学方法针对两种经典NPC标志物：巢蛋白和SOX 2。本文还描述了在条纹试验中从NPC获得足够的蛋白裂解物的方法。当蛋白裂解物进行Rac 1亲和沉淀时，在下拉样品中检测到Rac 1-GTP。此外，这些文章提供了活细胞成像数据，以更好地了解CXCL 12介导的细胞迁移和极化。
This data article contains three figures and three videos related to the research article entitled “Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization” Zhang et al. (2015) [1], which uses stripe assay to study mouse neural progenitor cell (NPC) migration and polarization. The current article describes the neurosphere method used to culture NPCs. NPCs in neurospheres and monolayer were characterized using immunocytochemistry method with antibodies against two classic NPC markers: nestin and SOX2. The article also describes method to obtain sufficient protein lysates from NPCs in the stripe assay. When protein lysates were subjected to Rac1 affinity precipitation, Rac1-GTP was detected in the pull-down samples. In addition, the articles provides live cell imaging data to better understand CXCL12-mediated cellular migration and polarization.