Hypoxia-conditioned media allows species-specific attraction of bone marrow stromal cells without need for recombinant proteins
Hypoxia-conditioned media allows species-specific attraction of bone marrow stromal cells without need for recombinant proteins
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DOI：
10.1186/1746-6148-10-56
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发表时间：
2014-03-04
影响因子：
2.6
通讯作者：
Roesen-Wolff, Angela
中科院分区：
文献类型：
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作者：
Gabrielyan, Anastasia;Knaak, Sven;Roesen-Wolff, Angela
背景资料：体内组织再生取决于干细胞迁移到受伤区域，它们分化成特定的细胞类型，以及它们与生成新组织所必需的其他细胞的相互作用。人骨髓间充质干细胞（Mesenchymal Stem Cells，MSCs）是骨髓基质细胞（Bone Marrow Stromal Cells，BMSCs）的一个亚群，在骨组织中具有迁移和分化为成骨细胞的能力。这可以通过重组生长因子和细胞因子来促进。在许多动物物种中，基因组序列、重组蛋白和/或抗体的可用性是有限的，因此需要新的方法来产生促进干细胞迁移到组织缺损区域的资源。在这里，我们使用人、羊、马和犬来源的骨髓基质细胞来产生低氧条件培养基（HCM），以吸引相应物种的BMSCs进行迁移测定。结果：我们表明HCM含有比血管内皮生长因子更有效的吸引子，因此可以用于许多动物物种，而不需要纯化的蛋白质。与蛋白质级分和/或重组蛋白的制备和纯化相比，HCM的产生容易且便宜。因此，HCM可以应用于大型动物（例如，G.羊、马、狗）用于将BMSC吸引到由肿瘤切除或创伤引起的组织缺损中。
Background: In vivo tissue regeneration depends on migration of stem cells into injured areas, their differentiation into specific cell types, and their interaction with other cells that are necessary to generate new tissue. Human mesenchymal stem cells, a subset of bone marrow stromal cells (BMSCs), can migrate and differentiate into osteoblasts in bone tissue. This can be facilitated by recombinant growth factors and cytokines. In many animal species, the availability of genomic sequences, recombinant proteins, and/or antibodies is limited so that new approaches are needed to generate resources that facilitate migration of stem cells into tissue defect areas. Here we used bone marrow stromal cells of human, ovine, equine, and canine origin to generate hypoxia-conditioned media (HCM) in order to attract BMSCs of the respective species in migration assays.Results: We show that HCM contain attractors even more potent than vascular endothelial growth factor and can therefore be used in many animal species without the need for purified proteins.Conclusion: Generation of HCM is easy and cheap compared to preparation and purification of protein fractions and/or recombinant proteins. Hence, HCM could be applied in large animals (e. g. sheep, horse, dogs) for attraction of BMSCs into tissue defects caused by tumor resection or trauma.