Functional characterization of peripheral blood dendritic cells and monocytes in systemic lupus erythematosus
Functional characterization of peripheral blood dendritic cells and monocytes in systemic lupus erythematosus
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DOI：
10.1007/s00296-010-1709-6
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发表时间：
2012-04-01
影响因子：
4
通讯作者：
Paiva, Artur
中科院分区：
文献类型：
--
作者：
Henriques, Ana;Ines, Luis;Paiva, Artur
为了更好地了解APCs在SLE中的功能活性，我们评估了外周血（PB）单核细胞和DC (tDC)，特别是髓细胞（mDC）和CD14（-/低）CD16(+) DC亚群产生促炎细胞因子（tnf - α, IL-1 β， IL-6和IL-12）的分布和功能能力，并将其与健康个体进行了比较。该研究在34名不同疾病活动度评分（SLEDAI）的SLE患者和13名年龄和性别匹配的健康对照组（NC）中进行。我们的研究结果显示，与非活动性疾病和NC患者相比，活动性疾病的SLE患者tDC的绝对数量和相对频率总体上有所下降，尽管这种下降似乎对PB DC亚群的分布没有影响。SLE患者的单核细胞数量与NC患者相似，而在没有刺激的情况下，单核细胞产生细胞因子的频率更高，每个细胞中每种细胞因子的数量也更高，这在活动性疾病患者中尤为明显。刺激后，我们观察到活动性SLE患者产生il -12单核细胞的频率更高。另一方面，我们发现在dc中产生细胞因子的CD14（-/低）CD16(+) dc的频率更高，每个细胞产生的细胞因子量更高，特别是在活动性疾病中。这些发现支持活动性SLE中APCs产生炎性细胞因子的增加，主要与CD14（-/低）CD16(+) DC亚群稳态的改变有关，这可能有助于解释这些细胞在疾病发病机制中的动态作用。
With the purpose of contributing to a better knowledge of the APCs functional activity in SLE, we evaluated the distribution and functional ability to produce pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6 and IL-12) of peripheral blood (PB) monocytes and DC (tDC), particularly myeloid (mDC) and CD14(-/low)CD16(+) DC subpopulations comparing them with those obtained from healthy individuals. The study was performed in 34 SLE patients with diverse disease activity scores (SLEDAI) and 13 healthy age- and sex-matched controls (NC). Our results show an overall decrease in absolute number and relative frequency of tDC in SLE patients with active disease when compared to those with inactive disease and NC, although this decrease did not seem to have an effect on the distribution of PB DC subsets. The monocytes number in SLE patients was similar to those found in NC, whereas a higher frequency of monocytes producing cytokines as well as the amount of each cytokine per cell found without stimulation was particularly observed in those patients with active disease. After stimulation, we observed a higher frequency of IL-12-producing monocytes in active SLE patients. On the other hand, we found among DCs higher frequencies of cytokine-producing CD14(-/low)CD16(+) DCs and a higher amount of cytokines produced per cell, particularly in active disease. These findings support an increased production of inflammatory cytokines by APCs in active SLE, mostly associated with alterations in CD14(-/low)CD16(+) DC subset homeostasis that might contribute to explain the dynamic role of these cells in disease pathogenesis.