Gradual development of the interferon-γ response of swine to porcine reproductive and respiratory syndrome virus infection or vaccination
Gradual development of the interferon-γ response of swine to porcine reproductive and respiratory syndrome virus infection or vaccination
复制标题
DOI：
10.1016/s0042-6822(03)00009-6
复制
发表时间：
2003-04-25
期刊：
影响因子：
3.7
通讯作者：
Zuckermann, FA
中科院分区：
文献类型：
--
作者：
Meier, WA;Galeota, J;Zuckermann, FA
猪感染强毒猪繁殖与呼吸综合征(PRRS)病毒后，可诱导快速、强健的抗体反应，主要由非中和抗体组成，并在大约3个月后减弱。相反，病毒特异性干扰素-γ分泌细胞(SC)在此期间在猪淋巴细胞群体中的初始起病时间保持在相当低的水平，然后频率逐渐增加，在感染后6个月趋于平稳。在用PRRS修饰的活病毒(MLV)疫苗免疫的猪身上，也观察到了类似的宿主体液和细胞免疫反应的极化。即使联合使用一种佐剂来增强对伪狂犬病(PR)MLV疫苗的免疫反应，也未能改变PRRS病毒特异性干扰素-伽马SC(主要由CD4/CD8pha双阳性记忆T细胞组成，少数是典型的CD4(-)/CD8Alphabeta(+)T细胞)的诱导和中和抗体的产生。此外，与灭活的PR病毒不同，无活性的PRRS病毒不会引起病毒中和抗体的产生。据推测，这种病原体的固有特性延迟了宿主干扰素-伽马反应的发展，并优先刺激了不能中和的抗体的合成。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
Infection of swine with virulent porcine reproductive and respiratory syndrome (PRRS) virus induced a rapid, robust antibody response that comprised predominantly nonneutralizing antibodies and waned after approximately 3 months. In contrast, the initial onset of virus-specific interferon (IFN)-gamma-secreting cells (SC) in the pig lymphocyte population remained at a fairly low level during this period and then increased gradually in frequency, plateauing at 6 months postinfection. A similar polarization of the host humoral and cellular immune responses was also observed in pigs immunized with a PRRS-modified live virus (MLV) vaccine. Even coadministration of an adjuvant that enhanced the immune response to a pseudorabies (PR) MLV vaccine failed to alter the induction of PRRS virus-specific IFN-gamma SC (comprising predominately CD4/CD8alpha double positive memory T cells with a minority being typical CD4(-)/CD8alphabeta(+) T cells) and the generation of neutralizing antibodies. Moreover, unlike inactivated PR virus, nonviable PRRS virus did not elicit virus-neutralizing antibody production. Presumably, an intrinsic property of this pathogen delays the development of the host IFN-gamma response and preferentially stimulates the synthesis of antibodies incapable of neutralization. (C) 2003 Elsevier Science (USA). All rights reserved.