TRANSPLANTATION OF HUMAN MELANOCYTES
TRANSPLANTATION OF HUMAN MELANOCYTES
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DOI：
10.1111/1523-1747.ep12470973
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发表时间：
1987-09-01
影响因子：
6.5
通讯作者：
MOELLMANN, GE
中科院分区：
文献类型：
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作者：
LERNER, AB;HALABAN, R;MOELLMANN, GE
在培养来自人类的色素细胞方面的最新进展使得有可能开始将自体黑素细胞移植到色素减退的皮肤区域中。在一个患有花斑病的病人身上，我们能够从背部正常色素沉着的皮肤的剃须活检中提取色素细胞，在培养中扩增细胞，然后将它们放回没有色素细胞的区域，并获得完美的效果。为了使细胞在培养物中生长，我们使用12-O-十四酰基-佛波醇-13-乙酸酯（TPA）以及霍乱毒素和异丁基甲基黄嘌呤。此时，可以用碱性成纤维细胞生长因子代替TPA。使用自体色素细胞培养物的过程为在皮肤的某些区域中没有黑色素细胞的患者的治疗的进一步进展打开了大门，如在白癜风或斑驳病患者中所见，或作为严重机械或热创伤的结果。
Recent advances in the culturing of pigment cells from human beings have made it possible to begin the transplantation of autologous melanocytes into areas of skin that are hypopigmented. In a patient with piebaldism we were able to take pigment cells from a shave biopsy of the normally pigmented skin of the back, expand the cells in culture, and return them to an area devoid of pigment cells and get a perfect take. To grow the cells in culture we used 12-O-tetradecanoyl-phorbol-13-acetate (TPA) as well as cholera toxin and isobutylmethyl xanthine. At this time, one can substitute basic fibroblast growth factor for TPA. The procedure of using autologous pigment cell cultures opens the door for further advances in the treatment of patients who do not have melanocytes in certain areas of the skin, as seen in patients with vitiligo or piebaldism, or as a consequence of severe mechanical or thermal trauma.