GeoChip-Based Analysis of Microbial Functional Gene Diversity in a Landfill Leachate-Contaminated Aquifer
GeoChip-Based Analysis of Microbial Functional Gene Diversity in a Landfill Leachate-Contaminated Aquifer
复制标题
基于 GeoChip 的垃圾渗滤液污染含水层微生物功能基因多样性分析
DOI：
10.1021/es300478j
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发表时间：
2012-06-05
影响因子：
11.4
通讯作者：
Zhou, Jizhong
中科院分区：
文献类型：
--
作者：
Lu, Zhenmei;He, Zhili;Zhou, Jizhong
采用功能基因芯片技术（GeoChip 3.0）对浅层垃圾渗滤液污染含水层中微生物群落的功能基因多样性和结构进行了研究。水样从八个威尔斯在同一含水层深度直接低于一个城市垃圾填埋场或沿着主要下降梯度地下水流路径。功能基因的丰富度和多样性直接低于垃圾填埋场和最近的井大大低于那些在downgradient威尔斯。Mantel检验和典范对应分析（CCA）表明，各种地球化学参数对地下微生物群落结构有显着的影响。也就是说，从无衬里的垃圾填埋场渗滤液的地下水微生物群落的多样性，组成，结构和功能潜力的影响，作为地下水pH值，硫酸盐，氨的浓度和溶解有机碳（DOC）的函数。历史的地球化学记录表明，所有采样的威尔斯长期接受渗滤液，并在微生物多样性的增加作为距离的功能，从垃圾填埋场是符合缓解渗滤液对地下水系统的影响，自然衰减机制。
The functional gene diversity and structure of microbial communities in a shallow landfill leachate-contaminated aquifer were assessed using a comprehensive functional gene array (GeoChip 3.0). Water samples were obtained from eight wells at the same aquifer depth immediately below a municipal landfill or along the predominant downgradient groundwater flowpath. Functional gene richness and diversity immediately below the landfill and the closest well were considerably lower than those in downgradient wells. Mantel tests and canonical correspondence analysis (CCA) suggested that various geochemical parameters had a significant impact on the subsurface microbial community structure. That is, leachate from the unlined landfill impacted the diversity, composition, structure, and functional potential of groundwater microbial communities as a function of groundwater pH, concentrations of sulfate, ammonia, and dissolved organic carbon (DOC). Historical geochemical records indicate that all sampled wells chronically received leachate, and the increase in microbial diversity as a function of distance from the landfill is consistent with mitigation of the impact of leachate on the groundwater system by natural attenuation mechanisms.