Vibrational Photoacoustic Tomography: Chemical Imaging beyond the Ballistic Regime
Vibrational Photoacoustic Tomography: Chemical Imaging beyond the Ballistic Regime
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DOI：
10.1021/jz401638e
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发表时间：
2013-10-03
影响因子：
5.7
通讯作者：
Cheng, Ji-Xin
中科院分区：
文献类型：
--
作者：
Rajian, Justin Rajesh;Li, Rui;Cheng, Ji-Xin
振动光声层析成像的概念证明与自制的拉曼激光器产生大于100毫焦耳的能量，每脉冲在1197 nm波长。我们用这个体系激发了C-H键的第二泛频跃迁。从放置在3cm厚鸡胸组织下的富含C-H的聚乙烯管体模获得了信噪比为2.5的振动光声信号。此外，我们记录了放置在5 mm鸡肉组织下的聚乙烯环的光声图像，具有良好的对比度。这一发展为在深层组织状态下进行无标记振动成像提供了新的机会。
Proof-of-concept of vibrational photoacoustic tomography is demonstrated with a home-built Raman laser generating greater than 100 mJ of energy per pulse at a 1197 nm wavelength. We employed this system for excitation of the second overtone transition of C-H bonds. The vibrational photoacoustic signal from a C-H-rich polyethylene tube phantom placed under 3 cm thick chicken breast tissue was obtained with a signal-to-noise ratio of 2.5. Further, we recorded a photoacoustic image of a polyethylene ring placed under 5 mm chicken tissue with excellent contrast. This development opens new opportunities of performing label-free vibrational imaging in the deep tissue regime.