Ultrahigh resolution photoacoustic microscopy via transient absorption
Ultrahigh resolution photoacoustic microscopy via transient absorption
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DOI：
10.1364/boe.1.000676
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发表时间：
2010-09-01
影响因子：
3.4
通讯作者：
Applegate, Brian E.
中科院分区：
文献类型：
--
作者：
Shelton, Ryan L.;Applegate, Brian E.
We have developed a novel, hybrid imaging modality, Transient Absorption Ultrasonic Microscopy (TAUM), which takes advantage of the optical nonlinearities afforded by transient absorption to achieve ultrahigh-resolution photoacoustic microscopy. The theoretical point spread function for TAUM is functionally equivalent to confocal and two-photon fluorescence microscopy, potentially enabling cellular/subcellular photoacoustic imaging. A prototype TAUM system was designed, built, and used to image a cross-section through several capillaries in the excised cheek pouch of a Syrian Hamster. The well-resolved capillaries in the TAUM image provided experimental evidence of the spatial resolution. These results suggest that TAUM has excellent potential for producing volumetric images with cellular/subcellular resolution in three dimensions deep inside living tissue. (C) 2010 Optical Society of America
