Humoral and cellular immune responses following vaccination with purified recombinant hemagglutinin from influenza A (H3N2) virus
Humoral and cellular immune responses following vaccination with purified recombinant hemagglutinin from influenza A (H3N2) virus
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DOI：
10.1093/infdis/175.2.342
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发表时间：
1997-02-01
影响因子：
6.4
通讯作者：
Wilkinson, BE
中科院分区：
文献类型：
--
作者：
Powers, DC;McElhaney, JE;Wilkinson, BE
成人用杆状病毒表达的纯化的重组A/Beijing/32/92（H3 N2）病毒血凝素（rHA）或生理盐水安慰剂免疫，并评价体液和体外细胞免疫应答。与安慰剂接受者的应答相比，疫苗接种者在接种后H3（Beijing/32）HA（H3）-特异性淋巴细胞增殖与白细胞介素（IL）-2、IL-10和干扰素-γ（LFN-gamma）产生，IL-10产生的平均增加IFN-γ（大于或等于20倍）和IL-2（10倍）的表达相对大于IFN-γ（4倍）或IL-4（10倍）的表达。在80%的rHA接受者中诱导血清H3抗体，并且抗体滴度的升高与IL-2、IL-10和IFN-γ浓度的变化显著相关。用rHA接种仅最低限度地增强抗流感病毒细胞毒性T淋巴细胞活性，这些数据表明，成人rHA免疫通过记忆B和T淋巴细胞激发了显著的回忆反应，并表明对疫苗接种的细胞因子反应具有T辅助细胞0型样特征。
Adults were immunized with either baculovirus-expressed, purified recombinant hemagglutinin (rHA) from influenza A/Beijing/32/92 (H3N2) virus or saline placebo and evaluated for humoral and in vitro cellular immune responses, Compared with responses in placebo recipients, vaccinees had greater postvaccination H3(Beijing/32) HA (H3)-Specific lymphoproliferation and interleukin (IL)-2, IL-10, and interferon-gamma (LFN-gamma) production, Mean increases in the production of IL-10 (greater than or equal to 20-fold) and IL-2 (10-fold) were relatively greater than that of IFN-gamma (4-fold) or IL-4 (no change), Serum H3 antibodies were induced in 80% of rHA recipients, and the rise in antibody titer was significantly correlated with changes in IL-2, IL-10, and IFN-gamma concentrations, Vaccination with rHA only minimally enhanced anti-influenza virus cytotoxic T lymphocyte activity, These data demonstrate that rHA immunization of adults elicits a significant recall response by memory B and T lymphocytes and suggest that the cytokine response to vaccination has a T helper cell type 0-like profile.