Biomarkers of disease severity in vivax malaria
Biomarkers of disease severity in vivax malaria
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DOI：
10.1007/s00436-021-07065-3
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发表时间：
2021-02-03
影响因子：
2
通讯作者：
Khan, Wajihullah
中科院分区：
文献类型：
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作者：
Aqeel, Sana;Naheda, Ansari;Khan, Wajihullah
在全世界范围内，已经观察到并确定了恶性疟原虫和间日疟原虫感染的严重并发症。尽管间日疟原虫感染尚未完全被认为是恶性疟疾，但最近许多研究报告出现了危及生命的并发症。具有出色灵敏度和可靠性的生物标志物的识别在疾病管理中发挥着重要作用。由于活性氧的产生，在疟疾中观察到急性炎症反应和氧化应激。由于寄生虫造成的损害，脂质和蛋白质氧化损伤是疾病严重程度的潜在生物标志物。我们试图找出蛋白质羰基化（PC）、脂质过氧化（LPO）和超氧化物歧化酶（SOD）是否足以作为严重间日疟疾的生物标志物。血液样本是在疟疾传播的雨季期间从阿里格尔穆斯林大学贾瓦哈拉尔尼赫鲁医学院就读的个人身上采集的。显微镜和快速诊断试剂盒被用作疟疾诊断的工具。共有 214 名受试者参加了该研究：30 名发热对照者和 184 名间日疟疾受试者。研究发现蛋白质羰基化和脂质过氧化与寄生虫计数和总抗氧化状态（TAS）直接相关。在严重间日疟疾患者中也观察到氧化应激增加。在复杂的病例中，尿酸和胆红素的水平也会升高。研究发现，与脂质过氧化相比，蛋白质羰基化是间日疟疾严重程度更可靠的指标。
Severe complications have been observed and established for Plasmodium falciparum as well as P. vivax infections worldwide. Although P. vivax infection is not fully acknowledged as malignant malaria, recently life-threatening complications have been reported to occur in many studies. The recognition of biomarkers with excellent sensitivity and reliability plays a prime role in disease management. Acute inflammatory response and oxidative stress are observed in malaria due to the production of reactive oxygen species. Lipid and protein oxidative injuries are prospective biomarkers for disease severity owing to the damage caused by the parasite. We have tried to find out whether protein carbonylation (PC), lipid peroxidation (LPO) and superoxide dismutase (SOD) could suffice as a biomarker for severe vivax malaria or not. Blood samples were collected from the individuals attending Jawaharlal Nehru Medical College of Aligarh Muslim University during the wet season of malaria transmission. Microscopy and rapid diagnostic kits were used as a tool for malaria diagnosis. A total of 214 subjects were enrolled for the study: 30 febrile controls and 184 subjects with vivax malaria. Protein carbonylation and lipid peroxidation were found to be directly associated with parasite count and total antioxidant status (TAS). Increase in oxidative stress was also observed in severe vivax malaria patients. Levels of uric acid and bilirubin too were raised in complicated cases. Protein carbonylation was found to be a more reliable indicator of vivax malaria severity than lipid peroxidation.