Helical metaphase chromatid coiling is conserved
Helical metaphase chromatid coiling is conserved
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DOI：
10.1101/2021.09.16.460607
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发表时间：
2021-09
期刊：
影响因子：
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通讯作者：
Ivona Kubalová;A. S. Câmara;P. Cápal;T. Beseda;J. Rouillard;G. Krause;Helena Toegelová;A. Himmelbach;N. Stein;A. Houben;J. Doležel;M. Mascher;H. Šimková;V. Schubert
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文献类型：
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作者：
Ivona Kubalová;A. S. Câmara;P. Cápal;T. Beseda;J. Rouillard;G. Krause;Helena Toegelová;A. Himmelbach;N. Stein;A. Houben;J. Doležel;M. Mascher;H. Šimková;V. Schubert
中期染色体的高阶组织已经争论了近 140 年。经典的光学和电子显微镜研究表明，染色单体由螺旋组织的染色质纤维（chromonemata）组成。最近还提出了非螺旋模型。我们使用尖端方法研究了大麦的染色体组织，并获得了螺旋排列的 400 nm 染色质纤维代表染色单体臂内染色质的证据。匝数与臂长呈正相关。转角大小和染色质密度向端粒方向减小。由于它们的特殊功能，着丝粒和核仁组织区域的螺旋组织被一些更细的直染色质纤维打断。与之前发表的数据的比较表明，中期染色单体臂的螺旋转动是大型真核染色体的保守特征。
The higher-order organization of metaphase chromosomes has been debated for almost 140 years. Classical light and electron microscopy studies suggested that chromatids are composed of helically organized chromatin fibers (chromonemata). Non-helical models were also recently proposed. We studied chromosome organization in barley using cutting-edge approaches and obtained evidence for a helically arranged 400-nm chromatin fiber representing the chromonema within chromatid arms. The number of turns is positively correlated with arm length. Turn size and chromatin density decrease towards the telomeres. Due to their specialized functions, the helical organization of centromeres and nucleolus-organizing regions is interrupted by several thinner, straight chromatin fibers. A comparison with previously published data indicates that the helical turning of metaphase chromatid arms is a conserved feature of large eukaryotic chromosomes.