EXTERNAL IONIC CONDITIONS, INTERNAL PH AND MOTILITY OF RAM AND BOAR SPERMATOZOA
EXTERNAL IONIC CONDITIONS, INTERNAL PH AND MOTILITY OF RAM AND BOAR SPERMATOZOA
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DOI：
10.1530/jrf.0.0980439
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发表时间：
1993-07-01
期刊：
影响因子：
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通讯作者：
DACHEUX, JL
中科院分区：
文献类型：
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作者：
GATTI, JL;CHEVRIER, C;DACHEUX, JL
本文研究了精巢、附睾、射出的公羊和公猪精子的内部pH和活力随外部离子组成的变化。用胺分布法测定细胞内pH，用活动细胞百分率和鞭毛节拍频率来表征细胞的运动能力。公猪和公羊精子在不同外pH值的介质中稀释后，其内部pH迅速向外pH变化。较高的外部Na+或K+浓度对平衡速率没有影响，对最终的内部pH值影响很小，排除了Na+-H+或K+-H+交换机制在这一过程中的作用。在两个物种中，观察到内外pH之间存在线性关系，但平衡不完全，表明存在复杂的调节机制。这一结果不受附睾成熟和射精的影响。公羊和猪睾丸精子在稀释后没有表现出运动增加，这表明内部pH的简单变化不足以触发运动。在较高的外部pH条件下，内部pH升高，启动了附睾猪精子的运动。公猪精子、附睾精子和绵羊精子的活力对外部pH的依赖性较小，受内部pH变化的影响很小。稀释后即刻K+或Na+对运动能力几乎没有影响。孵育1h后，运动量减少。在钠或钾溶液中维持活力表现出明显的外部最适pH。不含Na+和K+的培养基能较好地保存绵羊附睾液和射出精子在外加pH>；8时的活力，而在外加pH>；6时对猪射精有较好的保存效果。
Internal pH and motility of testicular, epididymal and ejaculated ram and boar spermatozoa were studied as a function of external ionic composition. Internal pH was estimated by the amine distribution method and motility was characterized by percentage of cells that were motile and flagellar beat frequency. Upon dilution in media at different external pH values, internal pH of boar and ram spermatozoa changed rapidly towards the external pH. High external concentrations of Na+ or K+ had no effect on the rate of equilibration and only a slight effect on the final internal pH value, ruling out a role of Na+-H+ or K+-H+ exchange mechanisms in this process. In both species, a linear relationship was observed between internal and external pH but equilibration was incomplete suggesting that there is a complex regulatory mechanism. This result was unaffected by epididymal maturation and ejaculation. Ram and boar testicular spermatozoa showed no increase in movement after dilution, suggesting that simple changes in internal pH are not a sufficient trigger for motility. At high external pH, internal pH increased and motility of epididymal boar spermatozoa was initiated. Motility of ejaculated boar spermatozoa, and epididymal and ejaculated ram spermatozoa was less dependent upon external pH and affected only very slightly by the internal pH changes. K+ or Na+ had almost no effect on motility just after dilution. After 1 h of incubation, movement decreased. Maintenance of motility in sodium or potassium showed a sharp external pH optimum. Media without Na+ and K+ allowed a better conservation of motility at external pH > 8 for ram epididymal and ejaculated spermatozoa and at external pH > 6 for boar ejaculated spermatozoa.