In-situ formation of growth-factor-loaded coacervate microparticle-embedded hydrogels for directing encapsulated stem cell fate.
In-situ formation of growth-factor-loaded coacervate microparticle-embedded hydrogels for directing encapsulated stem cell fate.
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DOI：
10.1002/adma.201405337
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发表时间：
2015-04-01
期刊：
影响因子：
--
通讯作者：
Alsberg E
中科院分区：
文献类型：
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作者：
Jeon O;Wolfson DW;Alsberg E
复凝聚是在水溶液中通过与两种带相反电荷的聚电解质之间的静电匹配相关的自发聚集而进行的液-液相分离。[1，2]尽管它们在医学和食品应用中具有潜力，但自1929年Bungenberg de Jong和Kruyt [3]引入该术语以来，复合凝聚层并没有得到大量研究，直到过去十年，该领域的兴趣在新的工程生物系统方面急剧增加。[4]复合凝聚层已被用于生物活性分子和多糖之间的直接复合，[5，6]生物活性分子或细胞的微米或纳米封装，[7，8]和颗粒的表面涂层，[9]由于其独特的物理化学特性，可以很容易地通过pH值，离子强度，电荷密度和相互作用分子的化学计量进行调节。[6，7，8，9]然而，这些系统通常需要细胞毒性表面活性剂和/或昂贵的设备。在温和条件下廉价形成细胞相容性凝聚体的能力，允许通过简单混合将细胞和生物活性因子区室化包封，这对于组织工程策略是有价值的，因为它允许控制它们的空间分布。[10]这种对细胞和生物活性因子位置的空间控制可以更好地促进对包封细胞命运的区域调节，这对于复杂组织的工程化至关重要。然而，据我们所知，还没有报道凝聚系统能够同时细胞
Complex coacervation is liquid-liquid phase separation in an aqueous solution by spontaneous aggregation associated with electrostatic matching between two oppositely charged polyelectrolytes.[1, 2] Despite their potential in medical and food applications, complex coacervates had not been heavily researched since the introduction of the term in 1929 by Bungenberg de Jong and Kruyt [3] until only the past decade when interest in the area increased dramatically in relation to new engineered biological systems.[4] Complex coacervates have been used for direct complexation between bioactive molecules and polysaccharides,[5, 6] micro-or nanoencapsulation of bioactive molecules or cells,[7, 8] and surface coating of particles,[9] due to their unique physicochemical characteristics that can be easily modulated by pH, ionic strength, charge density, and the stoichiometry of interacting molecules.[6, 7, 8, 9] However, these systems often require cytotoxic surfactants and/or expensive equipment. The ability to cheaply form cytocompatible coacervates under mild conditions that permit the compartmentalized encapsulation of cells and bioactive factors via simple mixing would be valuable for tissue engineering strategies, as it would allow for control over their spatial distribution.[10] This spatial control over the location of cells and bioactive factors may better facilitate the regional regulation of encapsulated cell fate, which is critical for the engineering of complex tissues. However, to the best of our knowledge, no coacervate system has been reported capable of simultaneous cell