Synergistic effect of ascorbic acid and collagen addition on the increase in type 2 collagen accumulation in cartilage-like MSC sheet.
Synergistic effect of ascorbic acid and collagen addition on the increase in type 2 collagen accumulation in cartilage-like MSC sheet.
复制标题
添加抗坏血酸和胶原蛋白对增加软骨样 MSC 片中 2 型胶原蛋白积累的协同作用。
DOI：
10.1007/s10616-015-9924-3
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发表时间：
2015
期刊：
影响因子：
2.2
通讯作者：
M. Takagi
中科院分区：
文献类型：
--
作者：
Y. Sato;H. Mera;D. Takahashi;T. Majima;N. Iwasaki;S. Wakitani;M. Takagi
为了提高人骨髓间充质干细胞制备的无支架软骨样细胞片中2型胶原的含量，研究了软骨形成培养基中几种添加剂的作用。添加高浓度（250 µg/ml）的2-磷酸抗坏血酸（VCP）和1型去端胶原（5 µg/ml）分别使2型胶原的积累增加了4倍和2倍。另一方面，抗氧化剂谷胱甘肽则没有这种效果。VCP和1型端胶原的协同作用导致2型胶原的积累水平增加了8倍。此外，2型胶原蛋白的基因表达水平增加，基质金属蛋白酶-13（MMP-13）的基因表达水平降低至对照组的约三分之一。在无支架软骨样细胞片中2型胶原积累的增加可能是由于MMP-13不仅促进了2型胶原的合成，而且抑制了2型胶原的降解。
Aiming to increase the content of type 2 collagen in scaffold-free cartilage-like cell sheets prepared using human bone marrow mesenchymal stem cells, the effect of several kinds of additives in a chondrogenic medium was investigated. Addition of ascorbic acid 2 phosphate (VCP) at a high concentration (250 µg/ml) and type 1 atelocollagen (5 µg/ml) increased the accumulation of type 2 collagen by fourfold and twofold, respectively. On the other hand, an antioxidant, glutathione showed no such effect. The synergistic effect of VCP and type 1 atelocollagen resulted in an eightfold increase in the accumulation level of type 2 collagen. Furthermore, the gene expression level of type 2 collagen increased and that of matrix metalloproteinase-13 (MMP-13) decreased to approximately one-third of the control. The increase in type 2 collagen accumulation in the scaffold-free cartilage-like cell sheet might be due to not only the enhancement of the synthesis but also the suppression of the degradation of type 2 collagen by MMP-13.