VOC breath profile in spontaneously breathing awake swine during Influenza A infection
VOC breath profile in spontaneously breathing awake swine during Influenza A infection
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DOI：
10.1038/s41598-018-33061-2
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发表时间：
2018-10-05
影响因子：
4.6
通讯作者：
Schubert, Jochen K.
中科院分区：
文献类型：
--
作者：
Traxler, Selina;Bischoff, Ann-Christin;Schubert, Jochen K.
流感是全球病毒性疾病最常见的原因之一。病毒检测需要测定上呼吸道中的流感病毒RNA。以这种方式进行有效的筛选是不可能的。呼吸中挥发性有机化合物（VOC）的分析有望用于无创和快速监测疾病进展。在高安全性条件下，在甲型流感的完整感染周期期间，重复分析了14头猪（3头对照动物和11头感染动物）的呼吸VOC特征。呼吸挥发性有机化合物通过针阱微萃取预浓缩，并通过气相色谱质谱法分析感染前，在病毒存在于鼻腔中，和恢复后。六种VOC可能与疾病进展相关：乙醛、丙醛、乙酸正丙酯、甲基丙烯酸甲酯、苯乙烯和1，1-二丙氧基丙烷。早在接种后第4天，当动物检测为甲型流感阳性时，对照动物和感染动物之间的差异是可能的。基于VOC的病毒感染信息可以早期发现甲型流感。由于VOC分析是完全非侵入性的，因此它具有大规模筛选目的的潜力。从某种角度来看，呼吸分析可能为人类医学、动物卫生控制或边境保护中的流感监测提供一种新的工具。
Influenza is one of the most common causes of virus diseases worldwide. Virus detection requires determination of Influenza RNA in the upper respiratory tract. Efficient screening is not possible in this way. Analysis of volatile organic compounds (VOCs) in breath holds promise for non-invasive and fast monitoring of disease progression. Breath VOC profiles of 14 (3 controls and 11 infected animals) swine were repeatedly analyzed during a complete infection cycle of Influenza A under high safety conditions. Breath VOCs were pre-concentrated by means of needle trap micro-extraction and analysed by gas chromatography mass spectrometry before infection, during virus presence in the nasal cavity, and after recovery. Six VOCs could be related to disease progression: acetaldehyde, propanal, n-propyl acetate, methyl methacrylate, styrene and 1,1-dipropoxypropane. As early as on day four after inoculation, when animals were tested positive for Influenza A, differentiation between control and infected animals was possible. VOC based information on virus infection could enable early detection of Influenza A. As VOC analysis is completely non-invasive it has potential for large scale screening purposes. In a perspective, breath analysis may offer a novel tool for Influenza monitoring in human medicine, animal health control or border protection.