SUMOylation facilitates the assembly of a Nuclear Factor-Y complex to enhance thermotolerance in Arabidopsis
SUMOylation facilitates the assembly of a Nuclear Factor-Y complex to enhance thermotolerance in Arabidopsis
复制标题
SUMOylation 促进核因子 Y 复合物的组装，以增强拟南芥的耐热性
DOI：
10.1111/jipb.13396
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发表时间：
2023-01-05
影响因子：
11.4
通讯作者：
Yang, Chengwei
中科院分区：
文献类型：
--
作者：
Huang, Junwen;Huang, Junjie;Yang, Chengwei
热应激（HS）对植物发育产生严重负面影响，已成为农业的主要威胁。植物中已进化出一种快速的转录调控级联反应来响应 HS。核因子-Y (NF-Y) 复合物对此机制至关重要，但 NF-Y 复合物的调控方式仍不清楚。在这项研究中，我们确定了拟南芥 HS 反应的中心调节因子 NF-YC10（NF-Y 亚基 C10）作为 SUMO 化（一种重要的翻译后修饰）的底物。生化分析表明，SUMO 连接酶 SIZ1（SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1）与 NF-YC10 相互作用，并在 HS 过程中增强其 SUMO 化。 NF-YC10 的 SUMO 化促进其与 NF-YB3 的相互作用以及核易位，其中 SUMO 相互作用基序 (SIM) 对于其与 NF-YC10 的有效结合至关重要。进一步的功能分析表明，NF-YC10 的 SUMO 化和 NF-YB3 的 SIM 对 HS 响应基因表达和植物耐热性至关重要。这些发现揭示了 HS 下 NF-Y 复合物组装中 SIZ1 介导的 NF-YC10 SUMO 化的作用，为植物非生物胁迫反应期间翻译后修饰在调节转录中的作用提供了新的见解。
Heat stress (HS) has serious negative effects on plant development and has become a major threat to agriculture. A rapid transcriptional regulatory cascade has evolved in plants in response to HS. Nuclear Factor-Y (NF-Y) complexes are critical for this mechanism, but how NF-Y complexes are regulated remains unclear. In this study, we identified NF-YC10 (NF-Y subunit C10), a central regulator of the HS response in Arabidopsis thaliana, as a substrate of SUMOylation, an important post-translational modification. Biochemical analysis showed that the SUMO ligase SIZ1 (SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1) interacts with NF-YC10 and enhances its SUMOylation during HS. The SUMOylation of NF-YC10 facilitates its interaction with and the nuclear translocation of NF-YB3, in which the SUMO interaction motif (SIM) is essential for its efficient association with NF-YC10. Further functional analysis indicated that the SUMOylation of NF-YC10 and the SIM of NF-YB3 are critical for HS-responsive gene expression and plant thermotolerance. These findings uncover a role for the SIZ1-mediated SUMOylation of NF-YC10 in NF-Y complex assembly under HS, providing new insights into the role of a post-translational modification in regulating transcription during abiotic stress responses in plants.