Association between sequence variants in cadmium-related genes and the cadmium accumulation trait in thermo-sensitive genic male sterile rice
Association between sequence variants in cadmium-related genes and the cadmium accumulation trait in thermo-sensitive genic male sterile rice
复制标题
镉相关基因序列变异与温敏基因雄性不育水稻镉积累性状的关联
DOI：
10.1270/jsbbs.18191
复制
发表时间：
2019-01-01
期刊：
影响因子：
2.4
通讯作者：
Li, Dongping
中科院分区：
文献类型：
--
作者：
Hao, Xiaohua;Wu, Canming;Li, Dongping
尽管杂交水稻品种的种植一直在增加，但当这种水稻在镉污染的环境中种植时，存在高水平镉（Cd）在籽粒中积累的风险。为了生产cd安全的杂交水稻，一种实用的方法是产生低cd积累的亲本系。在两系杂交育种中，温敏基因雄性不育系（TGMS）作为母本产生杂交种子。近年来，镉积累相关基因已被发现；然而，这些基因对水稻籽粒Cd积累的影响尚未见报道。本研究选择174个TGMS系进行Cd积累表型分析，并选择30个TGMS系（包括15个稳定的低Cd和15个高Cd系）进行单核苷酸多态性（SNP）基因分型和关联分析。利用logistic模型进行了关联研究，以确定七个候选Cd相关基因中Cd积累与可变位点之间的关系。在4个候选基因中发现9个序列变异位点与Cd积累显著相关，其中OsNRAMP1和OsNRAMP5中的两个位点为低Cd有利变异，分别解释了46.4%和22.6%的表型变异。这些基因座可作为鉴定水稻Cd积累特性和低Cd标记辅助育种的新分子标记。
Although cultivation of hybrid rice varieties has been increasing, there are risks that high levels of cadmium (Cd) will accumulate in grain when such rice is grown in Cd-polluted environments. To produce Cd-safe hybrid rice, one practical approach is the generation of low Cd-accumulating parental lines. In two-line hybrid breeding, thermosensitive genic male sterile (TGMS) lines function as female parents to yield hybrid seeds. Recently, Cd accumulation-related genes have been identified; however, the effect of these genes on Cd accumulation in the grains of TGMS lines has yet to be reported. Here, 174 TGMS lines were selected for Cd accumulation phenotyping, and 30 TGMS lines, including 15 stable low-Cd and 15 high-Cd lines, were selected for single-nucleotide polymorphism (SNP) genotyping and association analysis. Association studies were conducted to identify the relationship between Cd accumulation and variable sites within seven candidate Cd-associated genes using logistic models. Nine sequence variant sites in four of the candidate genes were found to be significantly associated with Cd accumulation, two of which in OsNRAMP1 and OsNRAMP5 are low-Cd favorable variants, explaining 46.4% and 22.6% of the phenotypic variation, respectively. These loci could be developed as new molecular markers for identification of Cd accumulation characteristics and low-Cd marker-assisted breeding.