Absorption and metabolism of isothiocyanates formed from broccoli glucosinolates: effects of BMI and daily consumption in a randomised clinical trial
Absorption and metabolism of isothiocyanates formed from broccoli glucosinolates: effects of BMI and daily consumption in a randomised clinical trial
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DOI：
10.1017/s0007114518002921
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发表时间：
2018-12-28
影响因子：
3.6
通讯作者：
Novotny, Janet A.
中科院分区：
文献类型：
--
作者：
Charron, Craig S.;Vinyard, Bryan T.;Novotny, Janet A.
萝卜硫素来源于西兰花中的萝卜硫苷，与抗癌作用有关。一项临床前研究表明，每天食用西兰花可能会增加可用于吸收的萝卜硫素和萝卜硫素代谢物的产生。本研究的目的是确定每天食用西兰花是否会改变西兰花芥子油苷中异硫氰酸酯的吸收和代谢。我们进行了一项平衡BMI和谷胱甘肽S-转移酶mu 1（GSTM 1）基因型的随机交叉人体研究（n 18），其中受试者每天食用不含西兰花（NB）的对照饮食16 d，或相同的饮食，每天食用200 g煮熟的西兰花和20 g生萝卜15 d（每日西兰花，DB）和100 g西兰花和10 g萝卜。在第17天，所有受试者食用200 g西兰花和20 g白萝卜。采集24小时的血浆和尿液，通过三重四极串联质谱分析萝卜硫烷及其代谢产物和芥酸。对于BMI >26 kg/m2（中位数）的受试者，NB饮食组的血浆AUC和总代谢产物的尿液排泄率高于DB饮食组，而对于BMI>26 kg/m2（中位数）的受试者，
Sulphoraphane originates from glucoraphanin in broccoli and is associated with anti-cancer effects. A preclinical study suggested that daily consumption of broccoli may increase the production of sulphoraphane and sulphoraphane metabolites available for absorption. The objective of this study was to determine whether daily broccoli consumption alters the absorption and metabolism of isothiocyanates derived from broccoli glucosinolates. We conducted a randomised cross-over human study (n 18) balanced for BMI and glutathione S-transferase mu 1 (GSTM1) genotype in which subjects consumed a control diet with no broccoli (NB) for 16 d or the same diet with 200 g of cooked broccoli and 20 g of raw daikon radish daily for 15 d (daily broccoli, DB) and 100 g of broccoli and 10 g of daikon radish on day 16. On day 17, all subjects consumed a meal of 200 g of broccoli and 20 g of daikon radish. Plasma and urine were collected for 24 h and analysed for sulphoraphane and metabolites of sulphoraphane and erucin by triple quadrupole tandem MS. For subjects with BMI >26 kg/m(2) (median), plasma AUC and urinary excretion rates of total metabolites were higher on the NB diet than on the DB diet, whereas for subjects with BMI